GDNF促进体外培养的大鼠C6胶质瘤细胞增殖的时效及量效关系的研究

目的观察外源性胶质细胞系源性神经营养因子（GDNF）促进大鼠C6胶质瘤细胞增殖的时效及量效关系，为深入研究GDNF对C6细胞的促增殖机制提供最佳的时效及量效作用点。方法将体外培养的C6细胞分为空白对照组、溶剂对照组和实验组（GDNF浓度分别为10、30、50、70、90μg／L，作用时间分别为12、24、48、72h），用MTT比色法检测不同浓度GDNF通过不同作用时间对C6细胞增殖情况的影响。结果浓度在50μg/L以上的GDNF作用24h后，C6细胞增殖水平升高，在48h时达到高峰而后趋于平稳或呈下降趋势，与对照组比差异有统计学意义。其中，70μg/LGDNF作用48h后，C6细胞的增殖率明显高于其他实验组。结论外源性GDNF促进体外培养的大鼠C6胶质瘤细胞增殖的最佳时效及量效作用点为浓度70μg/L、时间48h。

The research of the time-effect and dose-effect of GDNF on the proliferation of rat C6 glioma cells in vitro

Objective To investigate the relationship of the time - effect and dose - effect of exogenous glial cell - line derived neurotrophic factor （GDNF） on the proliferation of rat C6 glioma cells so as to explore the best action points of time - effect and dose - effect for further research into the mechanism of GDNF on the promotion of the proliferation of rat C6 glioma cells. Methods Rat C6 glioma ceils by conventional culture in vitro were divided into normal control groups, vehicle control groups and treatment groups （with the concentration of GDNF at 10, 30, 50, 70 and 90μg/L, treatment duration of 12, 24, 48 and 72 h, respectively）. The effects of GDNF on the proliferation of rat C6 glioma cells were detected by MTT colorimetric assay at different concentrations and time points. Results The level of proliferation of C6 cells increased at 24 h with GDNF at different concentrations （50, 70 and 90μg/L）, and reached its peak at 48 h and then showed a trend of decline, with differences of statistical significance. Among the groups, at the end of 48 h, the proliferation rate of C6 cells treated at the concentration of 70 μg/L GDNF was significantly higher than that in the other experimental groups. Conclusion 48 h and 70 p.g/L prove to be the optimal points of the time - effect and dose - effect of exogenous GDNF on the proliferation of rat C6 glioma cells in vitro.