| 间日疟原虫MSP1-19基因的克隆与表达 |
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| 引用本文： | 李光友,陈勇,夏惠,方强,刘丹,买月琴,贺文欣. 间日疟原虫MSP1-19基因的克隆与表达[J]. 蚌埠医学院学报, 2011, 36(9): 935-937 |
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| 作者姓名： | 李光友 陈勇 夏惠 方强 刘丹 买月琴 贺文欣 |
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| 作者单位： | 蚌埠医学院免疫学教研室,安徽,蚌埠,233030;蚌埠医学院病原生物学教研室,安徽,蚌埠,233030 |
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| 基金项目： | 卫生部寄生虫病预防与控制技术重点实验室开放课题资助项目 |
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| 摘 要： | 目的:构建间日疟原虫MSP1-19(Plasmodium vivax merozoite surface protein-1-19,Pv MSP1-19)基因克隆及表达的载体.方法:将MSP1-19基因克隆入原核表达载体pET28a中,构建重组表达载体.以重组载体转化大肠埃希菌BL21,在IPTG诱导下表达重组Pv M...
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| 关 键 词： | 间日疟 MSP1-19基因 原核表达 基因表达 |
Cloning and expression of Plasmodium vivax merozoite surface protein-1-19 gene |
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| LI Guang-you,CHEN Yong,XIA Hui,FANG Qiang,LIU Dan,MAI Yue-qin,HE Wen-xin. Cloning and expression of Plasmodium vivax merozoite surface protein-1-19 gene[J]. Journal of Bengbu Medical College, 2011, 36(9): 935-937 |
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| Authors: | LI Guang-you CHEN Yong XIA Hui FANG Qiang LIU Dan MAI Yue-qin HE Wen-xin |
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| Affiliation: | LI Guang-you1,CHEN Yong1,XIA Hui2,FANG Qiang2,LIU Dan2,MAI Yue-qin1,HE Wen-xin1(1.Department of Immunology,2.Department of Microbiology and Parasitology,Bengbu Medical College,Bengbu Anhui 233030,China) |
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| Abstract: | Objective:To construct a vector which clone and express Plasmodium vivax MSP1-19 Gene(Pv MSP1-19).Methods:The Pv MSP1-19 was cloned into the expression vector pET28a.The recombinant expression vector was transformed into E.coli,and the Pv MSP1-19 protein was expressed under IPTG induction.The recombinant protein was purified by affinity chromatography and the fusion protein was characterized by SDS-PAGE and Western blot.Results:The Pv MSP1-19 gene in plasmid pET28a was expressed in E.coli as a fusion protei... |
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| Keywords: | Plasmodium vivax merozoite surface protein-1-19 gene prokaryotic expression gene expression |
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