| 腺病毒介导的生长抑制基因4对ECV304细胞体外生长及分泌血管内皮生长因子的影响 |
| |
| 引用本文： | 张海峰,盛伟华,缪竞诚,陈卫东,杨吉成.腺病毒介导的生长抑制基因4对ECV304细胞体外生长及分泌血管内皮生长因子的影响[J].蚌埠医学院学报,2011,36(9):931-935. |
| |
| 作者姓名： | 张海峰 盛伟华 缪竞诚 陈卫东 杨吉成 |
| |
| 作者单位： | 1. 蚌埠医学院第一附属医院肾内科, 安徽蚌埠 233004;2. 苏州大学医学院细胞与分子生物学教研室, 江苏苏州 215123 |
| |
| 基金项目： | 安徽省高校省级自然科学研究资助项目 |
| |
| 摘 要： | 目的: 检测腺病毒介导的生长抑制基因4(adenovirus-mediated inhibitor of growth family member 4,Ad-ING4)感染后ECV304细胞体外生长及分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响,探讨ING4抑制肿瘤生长的可能机制。方法: 构建ING4腺病毒载体,用MTT法检测Ad-ING4感染后ECV304细胞体外生长影响,ELISA检测VEGF的分泌情况。结果: Ad-ING4感染后第3天和第4天ECV304细胞体外生长均受到抑制(P < 0.01),第4天时抑制率达63.6%,VEGF分泌受到抑制。结论: ING4能抑制ECV304细胞生长及VEGF分泌。Ad-ING4能抑制血管内皮细胞生长和血管的生成,从而可抑制体内肿瘤的生长。
|
| 关 键 词： | 腺病毒感染 生长抑制基因4 血管内皮生长因子 血管生成 |
| 收稿时间： | 2010-05-26 |
The effect of adenovirus-mediated inhibitor of growth family member 4 on growth and vascular endothelial growth factor secretion of ECV304 cells |
| |
| ZHANG Hai-feng,SHENG Wei-hua,MIAO Jing-cheng,CHEN Wei-dong,YANG Ji-cheng.The effect of adenovirus-mediated inhibitor of growth family member 4 on growth and vascular endothelial growth factor secretion of ECV304 cells[J].Journal of Bengbu Medical College,2011,36(9):931-935. |
| |
| Authors: | ZHANG Hai-feng SHENG Wei-hua MIAO Jing-cheng CHEN Wei-dong YANG Ji-cheng |
| |
| Institution: | 1. Department of Nephrology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004;2. Cell and Molecular Biology Instritute, Suzhou University School of Medicine, Suzhou Jiangsu 215123, China |
| |
| Abstract: | Objective:To interpret the possible anti-tumor mechanism of adenovirus-mediated inhibitor of growth family member 4(Ad-ING4) by testing the growth and vascular endothelial growth factor(VEGF) expression of ECV304 cells infected by Ad-ING4.Methods:Adenovirus vector of ING4 was constructed,then the growth of ECV304 cells was detected by MTT,and the VEGF expression of ECV304 cells infected by Ad-ING4 was detected by ELISA.Results:The VEGF expression of ECV304 cells infected by Ad-ING4 decreased,and the growth ... |
| |
| Keywords: | adenovirus infections inhibitor of growth family member 4 vascular endothelial growth factor angiogenesis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《蚌埠医学院学报》浏览原始摘要信息 |
| 点击此处可从《蚌埠医学院学报》下载免费的PDF全文 |
