| occludin蛋白与人乳腺癌细胞恶性增殖的相关性研究 |
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| 引用本文： | 赵锐,盛以芸,朱光能,丁淑琴,李友建,夏俊. occludin蛋白与人乳腺癌细胞恶性增殖的相关性研究[J]. 蚌埠医学院学报, 2011, 36(9): 925-930 |
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| 作者姓名： | 赵锐 盛以芸 朱光能 丁淑琴 李友建 夏俊 |
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| 作者单位： | 1. 蚌埠医学院临床检验诊断学实验中心, 安徽蚌埠 233030;2. 蚌埠医学院生物化学与分子生物学教研室, 安徽蚌埠 233030;3. 南昌大学第一附属医院病理科, 江西南昌 330006 |
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| 基金项目： | 安徽省教育厅自然科学研究资助项目 |
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| 摘 要： | 目的: 探讨occludin蛋白的表达与人乳腺癌细胞株MCF-7、MDA-MB-231、SKBR-3恶性增殖之间的关系。方法: 四甲基偶氮唑蓝法检测细胞增殖,流式细胞术检测细胞周期,FN蛋白黏附实验检测细胞黏附力,Transwell检测细胞迁移,RT-PCR检测occludin mRNA的表达,Western blot、细胞免疫化学技术检测occludin蛋白表达。结果: MCF-7细胞的增殖速度、S期所占比例、细胞黏附以及细胞迁移均小于MDA-MB-231和SKBR-3细胞(P < 0.05~P < 0.01),而MCF-7细胞中occludin mRNA及蛋白表达水平均高于MDA-MB-231和SKBR-3细胞(P < 0.01)。结论: occludin表达与人乳腺癌细胞恶性增殖、黏附及迁移呈一定的相关性。
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| 关 键 词： | occludin蛋白 乳腺癌细胞 细胞增殖 细胞周期 黏附 迁移 |
| 收稿时间： | 2011-03-28 |
The relevant research between expression of occludin and the malignancy multiplication in human breast cancer cells |
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| ZHAO rui,SHENG Yi-yun,ZHU Guang-neng,DING Shu-qin,LI You-jian,XIA Jun. The relevant research between expression of occludin and the malignancy multiplication in human breast cancer cells[J]. Journal of Bengbu Medical College, 2011, 36(9): 925-930 |
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| Authors: | ZHAO rui SHENG Yi-yun ZHU Guang-neng DING Shu-qin LI You-jian XIA Jun |
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| Affiliation: | 1. Research Center of Clinical Laboratory Science, Bengbu Medical College, Bengbu Anhui 233030;2. Department of Biochemistry and Molecular, Bengbu Medical College, Bengbu Anhui 233030;3. Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang Jiangxi 330006, China |
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| Abstract: | Objective:To study the relationship between the expression of occludin and malignancy multiplication in different human breast cancer cell lines MCF-7,MDA-MB-231 and SKBR-3.Methods:Cell proliferation was determined by MTT assay.The cell cycle was measured by flow cytometry.The adherence was detected by fibronectin protein.The migration of cells was detected by Transwell.The expression of occludin mRNA was examined by semi-quantitative RT-PCR.Western-blot and immunohistochemistry were used to detected the pr... |
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| Keywords: | occludin breast cancer cells cell proliferation cell cycle adherence migration |
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