急性胰腺炎大鼠肝功能的变化及阿司匹林等药物的影响

目的观察急性胰腺炎(AP)大鼠肝功能的变化及阿司匹林(ASP)等药物的影响,并探讨其机制.方法SD大鼠分为4组:正常对照组,AP对照组,AP ASP组,AP 汉防己甲素组(AP Tet组).采用牛磺脱氧胆酸钠复制AP模型,模型制成后10min,AP Tet组腹腔注入Tet(80mg/kg);AP ASP组经食管灌入ASP混悬液(125mg/kg).在各组动物被相应处理后1h、5h,分别取动物血浆测定其中淀粉酶(AMY)、谷丙转氨酶(ALT)和谷草转氨酶(AST)活性;取5h大鼠胰腺、肝脏等组织作病理检查.结果与AP对照组比较,ASP等药物组胰腺出血坏死和肝脏变性坏死程度减轻,血浆AMY、ALT和AST活性降低,P＜0.05或P＜0.01,各用药组之间差异不大.结论ASP、Tet可明显改善AP时胰腺、肝脏的组织及功能损伤.

Changes of the functional damage of liver in the course of AP and effect of medicines

Objective To investigate the change of hepatic function of rats with acute pancreatitis(AP), Also explore the effects of aspirin(ASP) and tetrandrine (Tet) on them. Methods The SD rats were divided into four groups, AP + ASP group(n=16), AP + Tet group(n = 16), AP + normal saline (NS) group(n = 16), and sham operation control (SO) group (n = 8). AP model was induced by a retrograde injection of 3% sodium deoxycholate into the pancreatic duct. The AP + Tet, the AP + NS, and the SO group got a intraperitoneal injection of Tet (80mg/kg)or NS, respectively. The AP + ASP group received an infusion of ASP(12. 5 mg /100g)by use of a nose-gastric catheter. At the selected time(1h, 5h) after the treatment, The levels of amylase( AMY), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the plasma of AP rats were measured by biochemical technic, and the pathologic changes of the pancreatic and the hepatic tissues were observed under a microscope. Results the levels of AMT, ALT and AST increased in the AP + NS group and decreased in the treated groups significantly(P<0.05, orP<0.01). The morphological lesions in the liver and pancreas were attenuated by these medicines. Conclusions ASP and Tet possessed protective effects on the morphological and functional damage of the pancreas and liver in the course of AP.