Comparative studies of collagens in normal and keratoconus corneas |
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| Authors: | D R Zimmermann R W Fischer K H Winterhalter R Witmer L Vaughan |
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| Affiliation: | 3. Augenklinik, Universitätsspital, CH-8091 Zürich, Switzerland;1. Department of Ophthalmic Oncology, Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA;2. Department of Pathology and Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA;1. Duke University, Durham, North Carolina;2. Department of Surgery, Duke Medicine, Durham, North Carolina;3. Department of Biostatistics, University of Nebraska Medical Center, Nebraska;4. Nebraska Medicine, Omaha, Nebraska;1. Department of Ophthalmology, St. Paul''s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;2. Catholic Institute for Visual Science, Seoul St. Mary''s Hospital, Seoul, Republic of Korea;1. School of Chemistry & Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332-0400, United States;2. Department of Ophthalmology, Mayo Clinic, Rochester, MN, 55905, United States;1. University of Virginia, Department of Chemistry, McCormick Road, Charlottesville, VA 22904-4319, USA;2. Department of Pathology, Health Sciences Center, University of Virginia, Charlottesville, VA 22908, USA |
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| Abstract: | In this paper we present strong evidence that the aberrations in keratoconus corneas are not directly related to alterations in collagen composition and distribution. This conclusion is based on comparative studies of collagen types I, III, IV, V and the recently described collagen types VI and VII in keratoconus and normal corneas. The data are derived from biochemical analysis of collagen fractions sequentially extracted with pepsin and sodium-dodecylsulphate, from amino acid analysis of hydrolysates of entire corneal tissues as well as from immunoblotting of the extracted collagens with specific antibodies. These antibodies were also used to examine the distribution of the collagens in immunofluorescence experiments on corneal sections. The yields of the collagen extractions were demonstrated to be age dependent but were not altered in keratoconus samples. Apart from one case associated with osteogenesis imperfecta type I, comparative studies of keratoconus and normal corneas showed no differences in collagen composition of the extracts. This was confirmed by amino acid analysis of tissue-hydrolysates. The distributions of collagen types I, III, IV, V, VI and VII in corneal sections were in general unchanged in keratoconus corneas, the only differences being in scar tissues observed in the Bowman layer of some keratoconus samples. |
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