| RT-PCR法观察光化学法损伤VSV核酸的动态变化 |
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| 引用本文： | 牛华,孙萍,周锡鹏,许金波. RT-PCR法观察光化学法损伤VSV核酸的动态变化[J]. 中国输血杂志, 2000, 13(1): 5-7 |
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| 作者姓名： | 牛华 孙萍 周锡鹏 许金波 |
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| 作者单位： | 军事医学院野战输血研究所,100850 |
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| 摘 要： | 目的 :了解光化学灭活病毒时 ,VSV核酸的动态变化。方法 :利用来源于VSV核衣壳蛋白基因的一对引物以RT PCR法检测VSV的核酸。结果 :RT PCR最大检测灵敏度为 1 8LgTCID50 ;在VSV光化学灭活过程中 ,RT PCR产物量在 5个灭活时间段上是不同的 ,随灭活时间的延长而减少。结论 :光化学法对VSV核酸损伤程度随病毒灭活时间的延长而增大。
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| 关 键 词： | 水疱性口膜炎病毒 光/亚甲基兰 病毒灭活 |
Observation on Dynamic Change in Photo inactivation-Damaged Nucleic Acid of VSV by RT-PCR Method |
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| Niu Hua,Sun Ping,Zhou Xipeng,et al.. Observation on Dynamic Change in Photo inactivation-Damaged Nucleic Acid of VSV by RT-PCR Method[J]. Chinese Journal of Blood Transfusion, 2000, 13(1): 5-7 |
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| Authors: | Niu Hua Sun Ping Zhou Xipeng et al. |
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| Affiliation: | Niu Hua,Sun Ping,Zhou Xipeng,et al.Institute of Transfusion,Beijing 100850 |
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| Abstract: | Objective:To understand the dynamic change in nucleic acid of VSV(vesicular stomatitis virus)in inactivating the virus by photochemistry.Methods:The nucleic acid of VSV was detected using a pair of primers originated from the gene of VSV nucleic env protein.Results:The maximal sensitivity of RT PCR method for VSV detection was 1.8Lg TCID 50 .In the process of photochemical inactivation of VSV,the amount of RT PCR product had varied at five time intervals of inactivation and decreased as the time of inactivation extended.Conclusion:The degree of VSV nucleic acid damage caused by photochemical method increases as the time of inactivation extend. |
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| Keywords: | RT PCR Vesicular stomatitis virus Light/Methylene blue Virus inactivation |
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