| 龙胆草对人黑素细胞增殖及黑素合成的影响 |
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| 引用本文： | 杨观招,解士海. 龙胆草对人黑素细胞增殖及黑素合成的影响[J]. 热带医学杂志, 2009, 9(8) |
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| 作者姓名： | 杨观招 解士海 |
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| 作者单位： | 广东省惠州市皮肤病医院,惠州,516001 |
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| 摘 要： | 目的探讨龙胆草对人黑素瘤细胞增殖及黑素合成的影响。方法采用MTT法测定龙胆草对人A375黑素瘤细胞增殖的影响,NaOH裂解法测定黑素合成,并与8-MOP的结果进行比较。结果0.1 mg/mL、0.2 mg/mL和0.5 mg/mL龙胆草对人A375黑素瘤细胞增殖无影响,0.1 mg/mL、0.2 mg/mL和0.5 mg/mL龙胆草对人A375黑素瘤细胞酪氨酸酶活性及黑素生成均有较强的剂量相关性激活作用,0.2 mg/mL及0.5 mg/mL龙胆草与对照组相比差异有统计学意义(P<0.01)。结论龙胆草对培养的人黑素瘤细胞酪氨酸酶活性及黑素生成有较强的剂量相关性激活作用。
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| 关 键 词： | 龙胆草 黑素细胞 酪氨酸酶 |
Effects of Radix Gentianae on Cell Proliferation and Melanin Synthesis of Cultured Human Melanoma Cells |
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| YANG Guan-zhao,XIE Shi-hai. Effects of Radix Gentianae on Cell Proliferation and Melanin Synthesis of Cultured Human Melanoma Cells[J]. Journal Of Tropical Medicine, 2009, 9(8) |
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| Authors: | YANG Guan-zhao XIE Shi-hai |
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| Affiliation: | Huizhou Hospital of Dermatology;Guangdong;Huizhou 516001;China |
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| Abstract: | Objective To study the effects of Radix Gentianae on cell proliferation and melanin synthesis of cultured human melanoma cells.Method MTT cytotoxicity assay and NaOH-assay were employed to measure the cell viability and melanin synthesis of human A375 melanoma cells;tyrosinase activity was measured by utilization of L-Dopa as the substrate.Furthermore,the effects of Radix Gentianae on human A375 melanoma cells were compared with 8-MOP.Result No influence was observed on the proliferation of human A375 cell ... |
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| Keywords: | Radix Gentianae melanocytes tyrosinase |
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