3种梅毒血清学方法筛查血液的效果评价

目的通过对梅毒3种方法的比较，选择适合血液筛查的最佳方法，防止梅毒经血液传播。方法用酶联免疫吸附试验双抗原夹心法（TPELISA）、甲本胺红不加热血清反应素试验（TRUST）、梅毒螺旋体颗粒凝集试验（TPPA）平衡检测献血者血样2000例，TP—ELISA和TRUST阳性标本用TPPA法确证。结果2000例标本，TP-ELISA法检测阳性11例，经TPPA法确证阳性8例；TRUST法检测阳性2例，经TPPA法确证均为阳性；TPPA法检测阳性8例；TRUST与TP-ELISA法比较差异有统计学意义（P〈0．05）；TRUST法和TP—ELISA法的灵敏度分别是25％、100％。结论TP—ELISA法检测梅毒具有灵敏度高、特异性好，适合于大批量样本的集中化和自动化检测等特点，是献血者血液筛查的理想方法。TRUST法灵敏度低，漏检率高，不适于献血者血液的筛查，TPPA法只适用于梅毒阳性标本的确证。

Efficiency evaluation on 3 serological methods for syphilis blood screening

Objective To compare the efficiency difference of 3 serological methods in syphilis blood SCreening,and then select the most proper method to prevent the transmission of syphilis via blood.Methods The samples of 2 000 blood donors were tested by TP-ELISA,TRUSlT and TPPA.Those positive samples detected by TP-ELISA and TRUST were confirmed by TPPA again.Results Of the 2 000 samples,11 samples were-TP-ELISA positive in which 8 samples were confirmed positivewith TPPA;2 samples were TRUSlT positive and both the two samples were confirmed positive withTPPA;and 8 cases were TPPA positive.There was obvious difference in detection rate between TRUST and TP-ELISA method(P