Optical imaging in vivo with a focus on paediatric disease: technical progress,current preclinical and clinical applications and future perspectives |
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Authors: | Joanna Napp Julia E Mathejczyk Frauke Alves |
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Institution: | 1.Department of Molecular Biology of Neuronal Signals,Max-Planck-Institute for Experimental Medicine,G?ttingen,Germany;2.Department of Hematology and Oncology,University Medical Center G?ttingen,G?ttingen,Germany |
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Abstract: | To obtain information on the occurrence and location of molecular events as well as to track target-specific probes such as
antibodies or peptides, drugs or even cells non-invasively over time, optical imaging (OI) technologies are increasingly applied.
Although OI strongly contributes to the advances made in preclinical research, it is so far, with the exception of optical
coherence tomography (OCT), only very sparingly applied in clinical settings. Nevertheless, as OI technologies evolve and
improve continuously and represent relatively inexpensive and harmful methods, their implementation as clinical tools for
the assessment of children disease is increasing. This review focuses on the current preclinical and clinical applications
as well as on the future potential of OI in the clinical routine. Herein, we summarize the development of different fluorescence
and bioluminescence imaging techniques for microscopic and macroscopic visualization of microstructures and biological processes.
In addition, we discuss advantages and limitations of optical probes with distinct mechanisms of target-detection as well
as of different bioluminescent reporter systems. Particular attention has been given to the use of near-infrared (NIR) fluorescent
probes enabling observation of molecular events in deeper tissue. |
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