| 抑癌基因nm23-H1诱导癌细胞凋亡及抗氧化作用的研究 |
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| 引用本文： | 杨瑞仪,张美英,赵利淦. 抑癌基因nm23-H1诱导癌细胞凋亡及抗氧化作用的研究[J]. 癌症, 2000, 19(5): 436-438 |
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| 作者姓名： | 杨瑞仪 张美英 赵利淦 |
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| 作者单位： | 暨南大学生物工程研究所!广东广州510630 |
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| 摘 要： | 目的：初步探讨ｎｍ２３－Ｈ１基因的抑癌机制。方法：实验将ｎｍ２３－Ｈ１基因经进行入人喉癌细胞Ｈｅｐ－２，使其在细胞中稳定表达。使用电镜观察细胞形态学变化，并测定抗氧化还开型谷胱苷肽（ＧＳＨ）浓度。结果：ｎｍ２３－Ｈ１转化的Ｈｅｐ－２细胞有核固缩、核分离等典型细胞凋亡现象，其ＧＳ浓度也有所升高（１６．９％）。结论：表明ｎｍ２３－Ｈ１具有诱导肿瘤细胞凋亡，提高肿瘤细胞抗氧化能力的作用。
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| 关 键 词： | nm23－H1基因 细胞凋亡 抗氧化 癌细胞 |
nm23-H_1 gene induces apoptosis and improves the antioxidation in tumor cell |
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| Yang Rui yi,Zhang Mei ying,Zhao Li gan Bioengineering Research Institute,Jinan University,Guangzhou,,P R China. nm23-H_1 gene induces apoptosis and improves the antioxidation in tumor cell[J]. Chinese journal of cancer, 2000, 19(5): 436-438 |
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| Authors: | Yang Rui yi Zhang Mei ying Zhao Li gan Bioengineering Research Institute Jinan University Guangzhou P R China |
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| Affiliation: | Yang Rui yi,Zhang Mei ying,Zhao Li gan Bioengineering Research Institute,Jinan University,Guangzhou,510632,P R China |
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| Abstract: | Objective:To investigate the effect of the human tumor suppressor gene nm23 H 1 Method:The human tumor suppressor gene nm23 H 1 was transfected in human laryngeal carcinoma cell Hep 2, and obtained the stable expressing cell line Results:Under the electron microscopy, the nuclear condensation and nuclear separation were abserved in the nm23 H 1 transfected Hep 2 cell Moreover, the antioxidant GSH concentration in nm23 H 1 transfected cell was higher than that in control cell Conclusion:The results suggest that the nm23 H 1 gene might cause apoptosis of tumor cell and improve the antioxidation of the tumor cell |
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| Keywords: | nm23 H 1 gene Apoptosis Antioxidantion |
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