| 一种改良的糖醛酸含量测定方法 |
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| 引用本文： | 林颖,黄琳娟,田庚元. 一种改良的糖醛酸含量测定方法[J]. 中草药, 1999, 30(11): 817-819 |
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| 作者姓名： | 林颖 黄琳娟 田庚元 |
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| 作者单位： | 中国科学院上海有机化学研究所,200023 |
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| 摘 要： | 用硫酸－咔唑法测定天然多糖化合物中糖醛酸含量时，发现中性糖对测定结果有影响。并提出相应的改进方法，即测定中性糖的吸收度，从样品的吸收度值减去中性糖的吸收度值，即为糖醛酸的吸收度值。
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| 关 键 词： | 硫酸－咔唑法 糖醛酸 多糖 测定 天然化合物 |
| 收稿时间： | 1999-03-02 |
| 修稿时间： | 1999-03-02 |
An Improved Method for the Quantitative Determination of Uronic Acid |
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| Lin Ying,Huang Linjuan and Tian Gengyuan. An Improved Method for the Quantitative Determination of Uronic Acid[J]. Chinese Traditional and Herbal Drugs, 1999, 30(11): 817-819 |
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| Authors: | Lin Ying Huang Linjuan Tian Gengyuan |
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| Affiliation: | Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032 |
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| Abstract: | The quantitative determination of uronic acid in natural polysaccharides by the traditional sulfuric acid-carbazole colorimetry, was found to be interfered by the presence of neutral sugars. Accordingly, an improved method was developed by determining the absorbance of the neutral sugar and substract this value from the absorbance of the sample to obtain the corrected value of uronic acid. |
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| Keywords: | sulfuric acid-carbazole colorimetry uronic acid polysaccharides |
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