Growth inhibitory effect of N-(phosphonacetyl)-L-aspartic acid on human myeloid leukemia-derived cell lines and modulation by dipyridamole |
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| Authors: | H Taguchi I Kubonishi I Miyoshi |
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| Affiliation: | Department of Internal Medicine, Kochi Medical School, Japan. |
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| Abstract: | Dipyridamole (DIP) inhibited the uptake of tritiated thymidine (TdR), deoxyuridine (UdR) and uridine (UR) in myeloid leukemia-derived cell lines, PL-21 and KCL-22. DIP also inhibited the growth of PL-21 (50% inhibitory concentrations, IC50, 15 microM) and KCL-22 (IC50, 3.0 microM). PALA inhibited the growth of PL-21 (IC50, 68 microM) and KCL-22 (IC50, 62 microM). The growth inhibition by PALA was significantly enhanced by the addition of 1 microM (PL-21) and 0.1 microM (KCL-22) of DIP. The inhibitory effect of PALA or PALA with DIP was completely abolished by the addition of 50 microM of UR in both cell cultures. Clinical evaluation of PALA in combination with DIP is warranted for the treatment of myeloid leukemia. |
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