加味参附汤对肾阳虚肺纤维化大鼠凋亡相关因子caspase-3和Bcl-2表达的影响

目的:观察加味参附汤对肾阳虚肺纤维化大鼠模型的病变程度及caspase-3和Bcl-2在模型大鼠肺内表达的影响。方法:大鼠60只随机分为正常组、模型组和干预组。模型组和干预组制作肾阳虚肺纤维化大鼠模型,造模成功后干预组给予加味参附汤治疗,常规石蜡切片,HE染色,观察肺泡炎及肺纤维化程度,免疫组织化学技术检测caspase-3和Bcl-2蛋白在各组大鼠肺组织中的表达。结果:caspase-3和Bcl-2主要在肺泡上皮细胞、肺泡巨噬细胞及浸润的炎症细胞中表达,第28天,模型组、干预组caspase-3和Bcl-2蛋白表达增强,均高于正常组(P0.05)。第42天,干预组大鼠细胞caspase-3表达较模型组降低(P0.05),而Bcl-2表达在干预组中增强,高于模型组(P0.05)。结论:在大鼠肾阳虚肺纤维化的发展过程中,肺组织细胞凋亡起重要作用,加味参附汤可用通过调控caspase-3和Bcl-2蛋白的表达而减少细胞凋亡,从而减缓肾阳虚肺纤维化的进展。

Effect of Modified Shenfu Decoction on Apoptosis-related Factors Caspase-3 and Bcl- 2 Expression in Pulmonary Fibrosis Rats with Kidney-yang Deficiency

Objective. To observe the effect of modified Shenfu Decoction on the pulmonary fibrosis pathological changes and caspase-3 and Bcl-2 expression levels in the pulmonary fibrosis rats of kid- ney-yang deficiency, and to explore the possible mechanism. Methods： Sixty Wistar rats were randomly divided into normal group, model group and intervention group. Kidney-yang deficiency of pulmonary fi- brosis was induced in the rats of the model group and intervention group, and then the intervention group was treated by Shenfu Decoction. After treatment, the rats of three groups were executed, and the lung was made into routine paraffin section. HE staining was performed for the observation of alveolitis and pulmonary fibrosis, and the expression of caspase-3 and Bcl-2 protein in rat lung tissue was detected by immunohistochemical method. Results. Obvious expression of caspase-3 and Bcl-2 was presented mainly in alveolar epithelial cells, pulmonary macrophages and infiltrated inflammatory cells. On day 28, model group and intervention group had higher expression of caspase-3 and Bcl-2 protein than that in the normal group（P 〈 0.05）., on day 42, caspase-3 expression in rats of intervention group was significantly lower than the model group（P 〈 0.05）, and the expression of Bcl-2 in the intervention group was en- hanced, higher than that of the model group（P 〈 0.05）. Conclusion： During the development of pulmonary fibrosis in kidney yang deficiency rat, the apoptosis of lung tissue cells play an important role, and modified Shenfu Decoction can slow down the progress by regulating caspase-3 and Bcl-2 protein expression to reduce apoptosis.