PON1 status evaluation in patients with chronic arterial occlusion of lower limbs due to atherosclerosis obliterans |
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Authors: | Magdalena P Kasprzak Maria Iskra Wac?aw Majewski Magdalena Budzyń-Napiera?a Bogna Gryszczyńska Krzysztof Strzy?ewski Jakub Kasprzak |
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Institution: | 1.Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland;2.Department of General and Vascular Surgery, Poznan University of Medical Sciences, Poznan, Poland;3.Department of Obstetrics and Gynecology with Pregnancy Pathology, Raszeja Hospital, Poznan, Poland |
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Abstract: | IntroductionHuman paraoxonase (PON1) is a calcium-dependent enzyme physically associated with HDL, and it is believed to contribute to the atheroprotective effect of HDL. The aim of the study was to evaluate PON1 status in patients with atherosclerosis obliterans as an effect of ischemia regarding its activity and phenotype distribution.Material and methodsThe study group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis obliterans (AO). The patients were divided into two groups according to the degree of ischemia: moderate (MI), and critical (CI). The ratio of the hydrolysis of salt-stimulated PON1 activity to the hydrolysis of phenylacetate was used to assign individuals to one of three possible phenotypes (low activity – A, medium activity – AB, high activity – B). It was observed that PON1 arylesterase activity was affected by ischemia of the lower limbs depending on its degree.ResultsThe odds ratio and the relative risk analysis showed that the patients with moderate ischemia are much more often characterized by phenotype A than by phenotype B. The low activity phenotype A occurs over twice as often in patients with chronic ischemia of the lower limbs as in individuals from the control group (OR = 2.125; 1.96 to 3.776, p = 0.0143).ConclusionsThis study presents the low activity phenotype A in relation to the risk of ischemia of the lower limbs due to atherosclerosis and shows the potentially important role of PON1 in conclusion of the process leading to intensification of ischemia degree. |
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Keywords: | low activity phenotype risk of ischemia arylesterase paraoxonase activity |
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