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| 地黄饮子改善自发性高血压大鼠进程中血管内皮功能的研究 | |
| 引用本文： | 王现珍,蒋嘉烨,罗珊珊,王勋,陆家凤,卞卡,可燕. 地黄饮子改善自发性高血压大鼠进程中血管内皮功能的研究[J]. 中国实验方剂学杂志, 2010, 16(14): 110-114 |
| 作者姓名： | 王现珍 蒋嘉烨 罗珊珊 王勋 陆家凤 卞卡 可燕 |
| 作者单位： | 1. 上海中医药大学教学实验中心,上海,201203 2. 上海高校一氧化氮与炎症医学研究院,上海,201203;美国德克萨斯大学休斯顿医学院综合生物及药理学系,德克萨斯大学分子医学研究所,休斯顿,TX,77030 3. 上海中医药大学教学实验中心,上海,201203;上海高校一氧化氮与炎症医学研究院,上海,201203 |
| 基金项目： | 国家科技部十一五支撑计划资助项目 |
| 摘 要： | 目的:探讨自发性高血压大鼠(SHR)高血压发展进程中,地黄饮子对其血管内皮功能的修复情况及作用机制研究。方法:54只雄性SHR随机分为3组:模型组、地黄饮子组和卡托普利组,7～24周龄ig地黄饮子(生药15.9 g.kg-1.d-1)和卡托普利(3.375 g.kg-1.d-1),停药观察至32周龄。18,24,32周龄时分别颈动脉插管测定大鼠血压值,血管等长张力实验检测离体肠系膜上动脉乙酰胆碱(Ach)依赖性舒张功能;Griess法检测血清NO 2-水平;实时荧光定量PCR(Real-time PCR)检测动脉组织白介素-1(IL-1),白介素-6(IL-6),肿瘤坏死因子-α(TNF-α)和iNOS mRNA)的表达。结果:地黄饮子降低SHR 18周龄血压,增加18,24周龄肠系膜上动脉Ach依赖性舒张功能和血清NO 2-含量,显著降低动脉组织IL-1,IL-6,TNF-α和iNOSmRNA表达;卡托普利降压作用优于地黄饮子,停药后作用消失,无改善肠系膜上动脉Ach依赖性舒张功能作用,增加血清24周龄NO 2-水平和动脉组织IL-1,IL-6 mRNA表达的同时降低TNF-α和iNOS表达。结论:地黄饮子能显著改善SHR肠系膜上动脉内皮功能,表现为较好的抗高血压作用。该作用可能与其抑制IL-1,IL-6,TNF-α和iNOS表达,改善血管微环境的炎症反应状态有关。 |
| 关 键 词： | 内皮功能障碍 炎症 地黄饮子 卡托普利 自发性高血压大鼠(SHR) 实时荧光定量PCR(Real-time |
| 收稿时间： | 2010-05-10 |
Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats: Role of Improving Endothelial Function |
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| WANG Xian-zhen,JIANG Jia-ye,LUO Shan-shan,WANG Xun,LU Jia-feng,BIAN Ka and KE Yan. Effect of Dihuang Yinzi on Hypertension Development in Spontaneously Hypertensive Rats: Role of Improving Endothelial Function[J]. China Journal of Experimental Traditional Medical Formulae, 2010, 16(14): 110-114 | |
| Authors: | WANG Xian-zhen JIANG Jia-ye LUO Shan-shan WANG Xun LU Jia-feng BIAN Ka KE Yan |
| Affiliation: | Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine, Shanghai 201203,China;E-institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine, Shanghai 201203, China;Department of Integrative Biology and Pharmacology, Institute of Molecular Medicine, University of Texas Medical School, Houston, Texas 77030, USA;Experiment Center for Teaching and Learning,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;E-institute of Shanghai Universities, Division of Nitric Oxide and Inflammatory Medicine, Shanghai 201203, China |
| Abstract: | |
| Keywords: | PCR) |
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