| 靶向牙周炎DNA疫苗免疫原性及免疫效能的实验研究 |
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| 引用本文： | 于飞,朱光勋,陈卫民. 靶向牙周炎DNA疫苗免疫原性及免疫效能的实验研究[J]. 临床口腔医学杂志, 2011, 27(7): 387-389. DOI: 10.3969/j.issn.1003-1634.2011.07.001 |
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| 作者姓名： | 于飞 朱光勋 陈卫民 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目(30700756) |
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| 摘 要： | 目的：检测树突状细胞靶向牙周炎DNA疫苗pCTLA4-FimA的免疫反应性,并评价其免疫保护能力。方法：BALB/c小鼠随机分为3组：pCTLA4-FimA鼻黏膜免疫组,非靶向牙周炎DNA疫苗pFimA鼻黏膜免疫组,pCI载体鼻黏膜免疫组。酶联免疫吸附实验检测血清及唾液中特异性抗FimA抗体水平。小鼠鼻黏膜途径免疫pCTLA4-Fi-mA、pFImA或pCI质粒,口腔接种Porphyromonas gingivalis,建立小鼠牙周炎模型,检测牙槽骨水平吸收程度。结果：与pFimA免疫组相比,pCTLA4-FimA免疫组诱导了显著增强的血清特异性IgG抗体和唾液特异性IgA抗体水平（P〈0.01）。与免疫前相比,三组小鼠牙槽骨均有吸收,其中pCTLA4-FimA免疫组牙槽骨水平吸收程度最低。结论：靶向牙周炎DNA疫苗pCTLA4-FimA能有效诱导机体产生特异性抗体免疫反应,并抑制牙周炎的发展,效果优于非靶向牙周炎DNA疫苗pFimA。
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| 关 键 词： | 牙龈卟啉单胞菌 菌毛蛋白 疫苗 DNA |
Immunogenicity and protective efficacy of a targeted DNA vaccine against FimA of porphyromonas gingivalis |
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| YU Fei,ZHU Guang-xun,CHEN Wei-min. Immunogenicity and protective efficacy of a targeted DNA vaccine against FimA of porphyromonas gingivalis[J]. Journal of Clinical Stomatology, 2011, 27(7): 387-389. DOI: 10.3969/j.issn.1003-1634.2011.07.001 |
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| Authors: | YU Fei ZHU Guang-xun CHEN Wei-min |
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| Affiliation: | YU Fei,ZHU Guang-xun,CHEN Wei-min.Center of Stomatology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei,Wuhan 430030,China. |
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| Abstract: | Objective:To evaluate the immunogenicity and protective efficacy of a dendritic cell targeted DNA vaccine pCTLA4-FimA.Method:Groups of three BALB/c mice,were immunized with pCTLA4-FimA,pFimA,or pCI by the intranasal route.Specific serum IgG and salivary IgA antibodies were detected by ELISA.Mice were immunized with pCTLA4-FimA,pFimA,or pCI intranasally,and then orally challenged with Porphyromonas gingivalis.Reduction of alveolar bone loss was observed.Result:pCTLA4-FimA induced increased specific serum and... |
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| Keywords: | Porphyromonas gingivalis FimA vaccine DNA |
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