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烧伤病人耐甲氧西林金黄色葡萄球菌及其肠毒素的检测和耐药性分析
引用本文:龙军,贾思远.烧伤病人耐甲氧西林金黄色葡萄球菌及其肠毒素的检测和耐药性分析[J].中国烧伤创疡杂志,2006,18(4):259-261.
作者姓名:龙军  贾思远
作者单位:1. 南方医科大学珠江医院检验科,广东,广州,510282
2. 南方医科大学珠江医院烧伤科,广东,广州,510282
基金项目:全军十五指令性课题(01L050)
摘    要:目的:评价乳胶结合实验,检测重症监护病房(ICU)耐甲氧西林金黄色葡萄球菌(MRSA)及其肠毒素(SE),并进行耐药性分析。方法:收集260株金黄色葡萄球菌临床分离株,通过药敏试验将其分为耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌(MSSA),用反向间接血凝试验(RPHA)检测金黄色葡萄球菌肠毒素。结果:MRSA产肠毒素为134株,MSSA产肠毒素为38株,MRSA产肠毒素率为100%,MSSA产肠毒素率为30%。结论:重症监护病房应重视MRSA的检测和金黄色葡萄球菌肠毒素的检测,合理使用广谱抗菌药物。

关 键 词:甲氧西林  葡萄球菌  耐药性  肠毒素
文章编号:1001-0726(2006)04-0259-03
收稿时间:2005-12-30
修稿时间:2006-03-16

Detection of methicillin resistant Staphylococcus aureus and its enterotoxin in burn patients
LONG Jun,JIA Si-yuan.Detection of methicillin resistant Staphylococcus aureus and its enterotoxin in burn patients[J].The Chinese Journal of Burns Wounds & Surface Ulcers,2006,18(4):259-261.
Authors:LONG Jun  JIA Si-yuan
Institution:1. Laboratory of Zhu-jiang Hospital, Nanfang Medical University, 510282, China; 2. Dept of Burns, Zhu-jiang Hospital, Nanfang Medical University, 510282, China
Abstract:Objective: To evaluate the result of latex fixation test for the detection of methicillin resistant Staphylococcus aureua and its enterotoxin in burn patients at intensive care unit. Method: 260 strains of Staphylococcus aureus were isolated and their resistance to methicilin was determined. Staphylococcus aureus enterotoxin was detected by reverse passive hemagglutination test. Result: 134 strains of methicilin resistant Staphylococcus aureus produced enterotoxin, accounted for 100% of the total. Only 38 strains of methicilin sensitive Staphylococcus aureus produced enterotoxin, accounted for 30% of the total. Conclusion: In intensive care unit, it is very important to detect drug resistance of Staphylococcus aureus and its enterotoxin production, so as rational application of the antibiotic can be achieved.
Keywords:Methicillin  Staphylococcus aureus  drug resistance  enterotoxin
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