川芎嗪抗家兔动脉粥样硬化作用

目的:研究川芎嗪抗动脉粥样硬化(AS)的药效学及作用机制.方法:随机将24只新西兰家兔分为4组,即正常饮食组、模型对照组、川芎嗪(75 mg·kg-1)组及川芎嗪(150 mg·kg-1)组.采用高脂饲料建立AS模型,12周后评价川芎嗪对主动脉斑块面积、内膜/中膜厚度比值、内膜泡沫细胞数、HE染色后主动脉形态学及血脂水平的影响.结果:川芎嗪(75,150mg·kg-1)与模型对照组比较,其斑块面积分别为(420.4±46.2) mm2,(310.2±12.3 )mm2,(663.2±38.9)mm2,P ＜0.01;内膜/中膜厚度比值(0.32±0.06),(0.24±0.15),(1.21±0.10)(P＜0.01)；降低内膜泡沫细胞数分别为(9.8±5.1)个/mm2,(5.3±1.2)个/mm2,(23.6±6.7)个/mm2 (P ＜0.01).HE染色后的光镜下,川芎嗪可减轻主动脉内膜增厚程度,降低泡沫细胞聚集,保持中膜和平滑肌结构基本完整,减少内皮细胞脱落,改善主动脉紊乱的形态结构；川芎嗪还可降低血脂水平而维持内皮细胞正常的生理功能.结论:川芎嗪靶向内皮细胞干预AS早期始动环节,抑制泡沫细胞形成产生抗AS作用.

Effect of Ligustrazine on Atherosclerosis in Rabbits

Objective: To investigate the antiatherosclerotic efficacy by ligustrazine (LIG). Method: Twenty-four rabbits were randomly divided into four groups: normal control group, model control group, LIG (75 mg·kg^-1) group and LIG (150 mg·kg^-1) group. In in vivo experiments, rabbits fed by high cholesterol diet have been used to evaluate the antiatherosclerotic efficacy of LIG (75, 150 mg·kg^-1) including plaque surface area in aorta, intima/media (I/M) ratios, foam cell amounts, serum lipid level of tolal cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) after twelve weeks. Result: Comparied with model control group, LIG could reduce the plaque surface area in aorta at the dose of 75, 150 mg·kg^-1 (420.4±46.2) mm² and (310.2±12.3) mm² vs (663.2±38.9) mm², P<0.01). I/M ratios and foam cell amounts were also decreased (P<0.01). Serum lipid level of TC, TG and LDL-C was depressed; however serum level of HDL-C had no changes. Conclusion: Antiatherosclerotic effects by LIG lie at endothelial cells.