蜈蚣提取液对局灶性脑缺血再灌注大鼠血浆vWF和TPO的影响

目的:研究蜈蚣提取液对局灶性脑缺血再灌注大鼠血浆血管假血友病因子(vWF)和血小板生成素(TPO)的影响.方法:将雄性SD大鼠采用线栓法建立大脑中动脉局灶性脑缺血再灌注大鼠模型,随机分为假手术组、模型组、蜈蚣提取液高、中、低剂量组(0.7,0.35,0.175 g·kg-1)和益气活血方组(8.54 g·kg-1),造模后连续ig给药14 d,采用ELASA法测定大鼠血浆vWF和TPO含量.结果:模型组大鼠血浆vWF和TPO含量明显高于假手术组,蜈蚣提取液高剂量组大鼠血浆vWF和TPO含量较模型组明显降低(P＜0.05或P＜0.01),益气活血方组大鼠血浆TPO含量较模型组明显降低(P＜0.01),益气活血方组大鼠血浆vWF较模型组降低但无显著性差异,蜈蚣提取液中、低剂量组大鼠血浆vWF和TPO含量较模型组降低,无显著性差异.结论:蜈蚣提取液能通过降低局灶性脑缺血再灌注大鼠血浆vWF和TPO的含量,改善脑缺血再灌注造成的损伤.

Effects of Centipede Extraction on vWF and TPO Expression in Blood Plasm after Focal Cerebral Ischemia-reperfusion Injury in Rats

Objective: To investigate the effects of centipede extraction on von willebrand disease fector(vWF) and thrombopoietin(TPO) expression after focal cerebral ischemia-reperfusion injury in rats. Method: Intraluminal thread occlusion of the middle cerebral artery(MCAO) was applied to establish the focal cerebral reperfusion model. The rats were randomly divided into six groups: sham-operative group, model group,high dose of centipede extraction group,middle dose of centipede extraction group,low dose of centipede extraction group and Yiqi Huoxue Recipe(YHR) group. The rats were reperfused again after occlusion of 2 hours. After intragastric administration of different drugs for 14 days, the blood plasma vWF and TPO were measured by enzyme-linked immunosorbent assay(ELISA). Result: The vWF and TPO levels were significantly up-regulated in rats after ischemia-reperfusion injury compared with sham-operative group. Compared with the model group, high dose of centipede extraction significantly ameliorated the vWF and TPO expression(P<0.05). Yiqi Huoxue Recipe administration could just decrease TPO level(P<0.05). The middle dose and low dose of centipede extraction reduced vWF and TPO levels without significant difference compared with model group. Conclusion: Centipede extraction could ameliorate cerebral ischemia-reperfusion symptoms via down-regulating the vWF and TPO levels.