| 两种硫酸长春新碱脂质体包封率测定方法的比较 |
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| 引用本文： | 李文静,杨志强,王杏林.两种硫酸长春新碱脂质体包封率测定方法的比较[J].中国实验方剂学杂志,2012,18(14):71-75. |
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| 作者姓名： | 李文静 杨志强 王杏林 |
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| 作者单位： | 1. 释药技术与药代动力学国家重点实验室,天津300193;天津中医药大学,天津300193
2. 释药技术与药代动力学国家重点实验室,天津300193;天津药物研究院,天津300193 |
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| 基金项目： | 国家重点基础研究发展计划项目(2010CB735602) |
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| 摘 要： | 目的:建立两种测定硫酸长春新碱脂质体包封率的方法,并对测定结果进行比较.方法:分别以葡聚糖凝胶Sephadex G-50柱和阳离子交换树脂柱分离硫酸长春新碱脂质体和游离药物,采用HPLC法测定药物含量,计算包封率,并用SPSS软件进行t检验.结果:两种方法测定不同药脂比的硫酸长春新碱脂质体包封率,测定结果无显著性差异.结论:葡聚糖凝胶柱法的建立需详细考察凝胶的种类对分离度的影响；阳离子交换树脂法分离度良好,分离速度快,为优选的方法.
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| 关 键 词： | pH梯度法 硫酸长春新碱脂质体 葡聚糖凝胶柱法 阳离子交换树脂法 包封率 |
| 收稿时间： | 2011/12/29 0:00:00 |
Comparison of Two Methods for Determination of Entrapment Efficiency of Vincristine Sulfate Liposome |
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| LI Wen-jing,YANG Zhi-qiang and WANG Xing-lin.Comparison of Two Methods for Determination of Entrapment Efficiency of Vincristine Sulfate Liposome[J].China Journal of Experimental Traditional Medical Formulae,2012,18(14):71-75. |
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| Authors: | LI Wen-jing YANG Zhi-qiang and WANG Xing-lin |
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| Institution: | State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin 300193,China;Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin 300193,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China;State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin 300193,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China |
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| Abstract: | Objective: Establish the two methods for the determination of entrapment efficiency of liposomes. Method: Sephadex G-50 and cation exchange resin were used to separate free vincristine sulfate from liposomes. The concentration of vincristine sulfate was determined by HPLC. The result was analyzed with SPSS. Result: The entrapment efficiency of the different drug loading liposome determined by the two methods was consistent. Conclusion: Both methods can be used to determine the entrapment efficiency of vincristine sulfate liposomes. |
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| Keywords: | pH gradient method vincristine sulfate liposome sephadex method cation exchange method entrapment efficiency |
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