| HPLC测定翼茎白粉藤中岩白菜素的含量 |
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| 引用本文： | 刘元,宋志钊,文志云. HPLC测定翼茎白粉藤中岩白菜素的含量[J]. 中国实验方剂学杂志, 2012, 18(14): 79-80 |
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| 作者姓名： | 刘元 宋志钊 文志云 |
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| 作者单位： | 广西中医药研究院,南宁,530022 |
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| 基金项目： | 广西科技攻关项目(桂科攻0992003-22);广西壮药质量标准(第二卷)研究项目(MZY2010049);广西区卫生厅自筹经费科研课题 (Z2011276) |
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| 摘 要： | 目的:建立翼茎白粉藤中岩白菜素的含量测定方法.方法:采用高效液相色谱法,以Appollo-C18柱(4.6 mm×250 mm,5μm)为分离柱,以甲醇-水(22∶78)为流动相,286 nm为检测波长,柱温为室温.结果:岩白菜素在10.6 ～ 169.6 mg·L-1,峰面积与其质量浓度呈良好线性关系(r =0.9992),平均回收率为100.5％,RSD 1.53％(n=6).结论:该方法简便、准确、重复性好,可作为该药材含量测定方法.
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| 关 键 词： | 翼茎白粉藤 高效液相色谱法 岩白菜素 |
| 收稿时间： | 2011-11-21 |
Determination of Bergenin in Cissus pteroclada by HPLC |
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| LIU Yuan,SONG Zhi-zhao and WEN Zhi-yun. Determination of Bergenin in Cissus pteroclada by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(14): 79-80 |
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| Authors: | LIU Yuan SONG Zhi-zhao WEN Zhi-yun |
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| Affiliation: | Guangxi Institute of Chinese Medicine & Pharmaceutical Scince,Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Scince,Nanning 530022, China;Guangxi Institute of Chinese Medicine & Pharmaceutical Scince,Nanning 530022, China |
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| Abstract: | Objective:To develop an HPLC method for detecting bergenin in Cissus pteroclada.Method: An Appollo-C18 column(4.6 mm×250 mm,5 μm)was used.The mobile phase consisted of methanol-water(22∶78).The detection wavelength was 286 nm and the column temperature was room temperature.Result: Bergenin showed a good linear relationship at the range of 10.6 to 169.6 mg·L-1,r=0.999 2.The recovery and RSD were 100.5% and 1.53%(n=6),respectively.Conclusion: This method was proved to be simple,accurate and repeatable to determine the content of bergenin in C.pteroclada. |
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| Keywords: | Cissus pteroclada HPLC bergenin |
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