神秘果混合果粉营养成分及其抗疲劳和免疫作用

目的:评价神秘果、杨桃、木瓜和南瓜等4种水果组成的混合果粉(SDMFP)的营养成分和其抗疲劳、调节免疫作用.方法:运用费林试剂热滴定法测定总糖含量,直接干燥法测定水分含量,酸水解法测定粗脂肪含量,凯氏定氮法测定粗蛋白含量,原子吸收法测定微量元素含量来分析SDMFP的营养成分.取昆明种雄性小鼠,随机分成空白对照组(生理盐水),SDMFP低、中、高剂量组(100,200,400 mg·kg-1 ·d-1),连续ig 14 d,通过测定小鼠负重游泳时间、血乳酸(BLA)、血清尿素氮(BUN)和肝糖原(LG)的含量变化探索SDMFP对小鼠抗疲劳能力影响,测定小鼠单核巨噬细胞吞噬功能和迟发型变态反应(DTH)分析SDMFP对小鼠免疫作用的影响.结果:SDMFP的营养成分为总糖42.12％±1.10％,粗脂肪1.97％±0.36％,粗蛋白0.33％±0.08％,水分11.31％ ±0.37％,K 5926.15 μg·g-1,Mg 826.75 μg·g -1,Ca 600.83 μg·g-1.与空白对照组比较,SDMFP高剂量组的小鼠负重游泳时间显著延长(P＜0.01),运动后血中BLA和BUN含量低于空白对照组,肌肉中的肝糖原含量高于空白对照组(P＜0.05),同时其巨噬细胞吞噬指数和胸腺、脾脏免疫器官质量得到明显的改善.结论:SDMFP能显著提高小鼠抗疲劳能力,并能一定程度增强细胞免疫作用.

Anti-fatigue and Immune Function by Nutritional Constituents from Synsepalum dulcificum Mixing Fruit Power

Objective:The anti-fatigue and immune properties by nutritional constituents from Synsepalum dulcificum mixing averrhoa carambola,carica papaya and pumpkin fruit powders(SDMFP) were evaluated.Method: The nutrition ingredients from SDMFP were analyzed as follows,the amount of total sugar using Fehling reagent hot titration,the content of moisture by directly dried,contents of crude fat and protein,were assayed by acid hydrolysis and Kieldahl method respectively,trace elements contents were determined through the atomic absorption method.The male mice were divided randomly into 4 groups: blank control group,low,medium,high group(SDMFP,100,200,400 mg·kg-1·d-1).The SDMFP were orally given to mice daily for 14 days,the loaded swimming time and some biochemistry indexes including blood lactic acid(BLA),hepatic glycogen(HG),and blood urea nitrogen(BUN) were tested to evaluate the anti-fatigue property.The function of macrophage cell and the delayed type hypersensitivity(DTH) were detected for the immune function.Result: The analysis result of SDMFP was: total sugar 42.12%,crude fat 1.97%,protein 0.33%,water 11.31%,and the content of K,Mg,Ca were 5 926.15,826.75,600.83 μg·g-1 respectively.Compared with the blank group,the mice in the high group(SDMFP,400 mg·kg-1·d-1) prolonged the loaded swimming time(P<0.01),reduced the blood lactic acid and blood urea nitrogen,and the content of hepatic glycogen were increased(P<0.05),the index of phagocytic macrophage cell and the weight of thymus and spleen were increased highly.Conclusion: The research results showed that the SDMFP could significantly enhance the ability of anti-fatigue and heighten the immune function of mice at a certain extent.