清开灵和葛根素注射液对清醒犬血压的影响

目的:清醒Beagle犬静脉滴注给予清开灵注射液或葛根素注射液,观察药物是否引起类过敏反应及其对血压的影响.方法:经手术在Beagle犬体内埋植植入子,手术恢复后用文献报道的类过敏阳性药compound 48/80和聚氧乙烯山梨糖醇酐单油酸酯(吐温-80)来验证该动物检测模型的可行性,然后分别经静脉滴注给予清开灵注射液或葛根素注射液,采用DataquestA.R.T.4.3系统连续监测给药过程中及给药后Beagle犬血压的变化并观察犬的体征变化.结果:类过敏阳性药compound 48/80和吐温-80给药后犬出现骚动、皮肤潮红、巩膜充血等类过敏症状,且血压出现不同程度的下降,证明清醒Beagle犬遥测系统检测类过敏反应模型成立.清开灵注射液1.2,2.3,11.5 mL·kg-1对犬血压均未见明显影响;0.24 mL·kg-1葛根素注射液组的血压变化不明显,而0.47 mL·kg-1葛根素注射液引起犬血压明显降低,平均动脉压在药后30 min时从药前的(115.35 ±7.73) mmHg降低到(91.74± 10.18) mmHg (P ＜0.01),收缩压从药前的(147.33±14.65) mmHg降低到(112.88 ±6.51) mmHg(P ＜0.01),但未见明显的体征变化.结论:清开灵注射液对清醒Beagle犬血压没有明显影响,葛根素注射液可引起清醒Beagle犬血压下降,且与剂量相关,可能是由其自身的药理作用引起的,初步判断清开灵注射液和葛根素注射液没有引起清醒Beagle犬发生类过敏反应.

Effects of Qingkailing and Puerarin Injection on Blood Pressure in Conscious Dogs

Objective: To investigate the effects of Qingkailing and Puerarin injection on blood pressure in conscious dogs. Method: The sensor was implanted in abdomen of conscious dogs. Qingkailing and Puerarin injection were intravenously injected after the dogs recovered. The change of blood pressure in dogs was monitored with Dataquest A.R.T.4.3 system. Result: After the administration of Compound 48/80 and Tween-80,blood pressure of dogs was decreased,which was statistically significant.Changes of blood pressure in dogs were not apparent after the administration of Qingkailing injection of 1.2,2.3,11.5 mL·kg^-1 or Puerarin injection of 0.24 mL·kg^-1.Blood pressure in dogs was decreased after the administration of Puerarin injection of 0.47 mL·kg^-1.Average blood pressure dropped from (115.35±7.73) mmHg to (91.74 ±10.18) mmHg(P<0.01) and systolic blood pressure dropped from (147.33±14.65) mmHg to (112.88 ±6.51) mmHg(P<0.01) thirty minutes later. Conclusion: Qingkailing injection has no effect on blood pressure in conscious dogs,the reduction of blood pressure in dogs caused by Puerarin injection is related to dosage,which may be caused by the pharmacological actions of itself.We preliminary conclude that Qingkailing injection and Puerarin injection cannot cause anaphylactoid reaction in conscious Beagle dogs.