| 生物杀虫剂—茶蚕颗粒体病毒的细胞学研究 |
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| 引用本文： | 黄晓沐,蒋家璞,姜宗荣,刘茜,赵恒奎.生物杀虫剂—茶蚕颗粒体病毒的细胞学研究[J].安徽预防医学杂志,1995(1). |
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| 作者姓名： | 黄晓沐 蒋家璞 姜宗荣 刘茜 赵恒奎 |
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| 作者单位： | 安徽省卫生防疫站 |
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| 摘 要： | 用生物杀虫剂茶蚕颗粒体病毒接种中国仓鼠肺细胞（ＣＨＬ）和鸡胚细胞未能引起细胞病变，茶蚕颗粒病毒亦不能在其中增殖，接种病毒颗粒后５天的原代细胞和盲代３代的细胞超薄切片的电镜观察未发现病毒包涵体。ＣＨＬ细胞的空斑实验证实了该病毒未感染ＣＨＬ细胞，但进行的ＣＨＬ细胞微核试验出现可疑阳性结果，提示茶蚕颗粒体病毒制剂中某些物质可能会引起遗传物质的改变
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| 关 键 词： | 茶蚕颗粒体病毒，中国仓鼠肺细胞，鸡胚细胞，空斑实验，微核试验 |
CYTOGENETICS STUDY ON ANDRACA BIPUNCTATA GRANULOSIS VIRUS |
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| Huang xian mo,etal..CYTOGENETICS STUDY ON ANDRACA BIPUNCTATA GRANULOSIS VIRUS[J].Anhui Journal of Preventive Medicine,1995(1). |
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| Authors: | Huang xian mo etal |
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| Institution: | Anhui Anti-Epidemic Station |
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| Abstract: | The cytogenetics study on Andraca Bipunctata Granulosis Virus(ADGV) aspecial biogenic insecticide,has been conducted by means of cultured with Chinese hamster Lung Cells (CHL) and embryo cells of chicken.The empty spot test in CHL was negative and the micronucleus test in CHL was double.Neither pathological change nor proliferation of cells was observed, Inclusion body ofvirus was not discovered both in originaly cultured cells after 5 days inoculating and blinding three generation cells under the electron microscope.These results suggest that the ADGV was safety in cytogenetics as a biogenic insecticide. |
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| Keywords: | CHL ADGV embryo lells of chicken empty spot test micronucleus test |
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