Notch-1调控视网膜前体细胞向视网膜神经节细胞分化

目的研究Notch-1对视网膜前体细胞(RPC)向视网膜神经节细胞(RGC)分化的调控作用。方法分离培养胚胎14 d龄Sprague-Dawley大鼠的RPC，实验组和对照组分别用含有Notch-1反义寡核苷酸链和无关序列寡核苷酸链的培养液进行诱导分化14 d，倒置相差显微镜每天观察细胞的生长和分化情况，Thy1.1标记RGC并进行计数。结果实验组和对照组的RPC都能分化为多种视网膜细胞类型，包括Thy1.1阳性的RGC，但两组RPC向RGC分化的百分比不同。实验组和对照组RGC的百分比分别为(16.57±4.31)%和(31.19±6.90)%，两组比较差异有统计学意义（t=9.84,P＜0.001）。结论Notch-1对RPC的分化具有负向调控作用，阻断Notch-1能促进RPC向RGC分化。(中华眼底病杂志, 2007, 23: 101-103)

Regulating effect of Notch-1 on retinal progenitor cells differentiating into retinal ganglion cells

ObjectiveTo investigate the regulating effect of Notch-1 on retinal progenitor cells (RPC) differentiating into retinal ganglion cells (RGC). MethodsRPC of 14-day embryonic SD rats were induced and differentiated in the culture medium with Notch-1 antisense oligonucleotides (experimental group) or missense oligonucleotides (control group) for 14 days. The condition of growth and differentiation of the cells were observed daily under the phase-contrast microscope. RGC were identified by Thy1.1 immunocytochemistry methods and the cellular number was counted. ResultsRPC in both of the two groups differentiated into various retinal cells, including Thy1.1-positive RGC. The percentage of RGC derived from RPC was 31.19%±6.90% in experimental group and 16.57%±4.31% in control group, and the difference was statistically significant (t=9.84，P<0.001). ConclusionNotch-1 may down-regulate the differentiation of RPC, and the inhibition of Notch-1 may promote RPC differentiating into RGC. (Chin J Ocul Fundus Dis, 2007, 23: 101-103)