移植含嗅鞘细胞和体外溃变的周围神经对成年大鼠视网膜神经节细胞轴突再生作用的比较

目的比较含嗅鞘细胞（OEC）或（和）体外溃变的周围神经移植对成年大鼠视网膜神经节细胞（RGC）轴突再生的影响。方法将24只成年雄性Sprague-Dawley大鼠随机分为4组，每组各6只大鼠：A组（周围神经对照组）：将取出的一段自体坐骨神经与眶内切断的左侧视神经近侧断端吻合；B组（OEC注入周围神经组）：自取出的坐骨神经两端注入10 μl OEC悬液后移植于视神经断端。C组（周围神经体外溃变组）：将取出的坐骨神经在体外单独培养5 d后植于视神经断端；D组（OEC-周围神经共培养组）：将取出的坐骨神经与OEC共培养5d后植于视神经断端。移植术后4周处死动物，计数各组以5%荧光金逆行标记的再生RGC数量。结果B、C、D三组RGC均数1481±268、1235±266和1464±285显著高于A组799±109（P值分别为0.0002、0.0010和0.0003）；B、C、D三组间差异无统计学意义（P值分别为0.3644、0.9167和0.4344）。结论OEC具有促进RGC轴突在新鲜周围神经移植物中再生的作用，但这种作用与体外溃变的周围神经相比无明显差异，二者亦无协同作用。（中华眼底病杂志，2007，23：130-132）

Comparison of the effects of olfactory ensheathing cell-containing and pre-degenerated peripheral nerve transplantation on axonal regeneration of axotomized retinal ganglion cells in adult rats

ObjectiveTo compare the effects of olfactory ensheathing cell (OEC)-containing and pre-degenerated peripheral nerve (PN) transplantation on the axonal regeneration of axotomized retinal ganglion cells (RGC) in adult rats.MethodsTwenty-four Sprague-Dawley rats were randomly divided into 4 groups with 6 rats in each group. A segment of the normal (group A) or 10μl-OEC-injected (group B) autogenetic sciatic nerve was sutured onto the ocular stump of the left transected optic nerve (ON). In another 2 groups, the removed sciatic nerve was cultured (group C) or co-cultured with OEC (group D) in vitro for 5 days before transplantation. All animals were executed 4 weeks after transplantation, and the number of Fluoro-gold labeled RGC in each group was counted.ResultsThe averages of regenerating RGC in group B (1481±268), C (1235±266) and D (1464±285) were significantly higher than that in group A (799±109; P=0.0002, 0.0010 and 0.0003, respectively). No significant difference was found among group B, C and D (P=0.3644, 0.9167 and 0.4344). ConclusionOEC can promote the axonal regeneration of axotomized RGC in fresh PN graft, which doesn′t differ much from the effect of the pre-degenerated PN graft. No additive effect of OEC and the pre-degenerated PN graft can be detected.(Chin J Ocul Fundus Dis, 2007, 23: 130-132)