不同温度条件下唾液斑酯酶表型的可测期限

目的　探讨不同温度条件下滤纸唾液斑酯酶的可测期限。方法　应用聚丙烯酰胺凝胶盘状电泳及固蓝RR染色方法 ,检测 4℃、室温 (18～ 2 5℃ )及 37℃条件下保存的滤纸上唾液斑酯酶表型的可测期限。结果　滤纸唾液斑 4℃保存 12周 ,室温保存 6周 ,37℃保存 5天 ,均可正确检出酯酶表型。唾液斑酯酶的三种表型F、FS和S ,F和S表型唾液斑在 4℃保存12周 ,室温保存 6周 ,37℃保存 5天 ,均可正确检出 ;FS表型唾液斑在 4℃保存 13周 ,室温保存 7周 ,37℃保存 6天 ,均可正确检出。结论　 4℃保存的唾液斑酯酶表型的可测期限比室温及 37℃条件下保存的长。在同样温度条件下保存的唾液斑 ,FS表型的可测期限比F和S表型的长

Possible detected period of phenotypes of esterases in saliva stains in different temperatures

Objective To research the possible detected period of esterases in saliva stains in different temperatures.Methods The saliva stain samples on filter paper kept at 4℃, room temperature (18～25℃) or 37℃ were detected by disc electrophoresis and fast blue RR staining assay.Results The phenotypes of saliva stain esterase on filter paper could be typed correctly at 4℃ for12 weeks, at room temperature for 6 weeks and at 37℃ for 5 days.The F and S phenotypes of saliva stain esterase could be typed correctly at 4℃ for 12 weeks, at room temperature for 6 weeks and at 37℃ for 5 days; The FS phenotypes of saliva stain esterase could be typed correctly at 4℃ for13 weeks, at room temperature for 7 weeks and at 37℃ for 6 days. Conclusion The possible detected period of phenotypes of the esterase on filter paper was longer than that at room temperature and at 37℃.The possible detected period of FS phenotype was longer than F and S phenotypes at the same temperature