PTEN 和hMSH2蛋白的表达在胃癌发生中的作用

目的]通过探讨胃癌组织PTEN 和hMSH2蛋白的表达的相互关系,分析二者表达在胃癌发生中的意义.方法]用链霉素-生物素蛋白-过氧化酶法（SP法）检测63例胃癌组织及32例非癌胃黏膜组织中PTEN 和hMSH2蛋白的表达情况.结果]PTEN蛋白在胃癌组织中的阳性表达率（61.9%）（39/63）显著低于非癌胃黏膜组织（90.6%）（29/32）（P＜0.05）,在高中分化腺癌中的阳性表达率（57.9%）（11/19）低于低分化腺癌（72.4%）（21/29）和黏液癌（60%）（9/15）,但无显著性差异（P＞0.05）;hMSH2蛋白在胃癌组织中的阳性表达率（79.4%）（50/63）显著高于非癌胃黏膜组织（53.1%）（17/32）（P＜0.05）,但在高中分化腺癌（73.7%）（14/19）、低分化腺癌（82.8%）（24/29）和黏液癌（80%）（12/15）中的阳性表达率无显著性差异（P＞0.05）.PTEN和hMSH2蛋白在胃癌组织中的表达无明显相关关系（r＝-0.158,P＞0.05）.结论]胃癌发生时可出现PTEN蛋白表达下调,伴hMSH2蛋白的表达上调.

Roles of PTEN and hMSH2 expressions in gastric carcinogenesis

Objective] To examine the expressions of PTEN and hMSH2 in gastric carcinoma and study the roles of their expressions in gastric carcinogenesis.Method]Streptavidin-biotion-peroxidase method(SP) was used to detect the expressions of PTEN and hMSH2 in gastric carcinoma and normal mucosa as control.Results] The positive rate of PTEN expression in gastric carcinoma being 61.9% (39/63) was significantly lower than that in gastric normal mucosa being 90.6%(29/32)(P<0.05),and the rate in well & middle differentiated group being 57.9%(11/19) was lower than those in poor differentiated group being 72.4%(21/29) and mucoid carcinoma group being 60%(9/15),but there was no significant difference between them(P>0.05).The positive rate of hMSH2 expression in gastric carcinoma being 79.4%(50/63) was significantly higher than that in gastric normal mucosa being(53.1%(17/32))(P<0.05).The expression of hMSH2 was not related to the differentiation of gastric carcinoma(P>0.05).The expressions of PTEN were not related to the expressions of hMSH2 in gastric carcinoma and normal mucosa.Conclusion] The absence of PTEN expression with the up-regulated hMSH2 expression related to gastric carcinogenesis.It may be helpful for diagnosing gastric carcinoma to detect the expressions of the two gene products.