Hepatitis B virus subgenotype C2- and B2-associated
mutation patterns may be responsible for liver cirrhosis and hepatocellular
carcinoma,respectively |
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Authors: | YM Chen SH Wu CN Qiu DJ Yu XJ Wang |
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Institution: | Affiliated Hangzhou Hospital, Nanjing Medical University, Department of Laboratory Medicine, Hangzhou, China, Department of Laboratory Medicine, Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China |
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Abstract: | The objective of this study was to examine hepatitis B virus (HBV) subgenotypes
and mutations in enhancer II, basal core promoter, and precore regions of HBV in
relation to risks of liver cirrhosis (LC) and hepatocellular carcinoma (HCC) in
Southeast China. A case-control study was performed, including chronic hepatitis
B (CHB; n=125), LC (n=120), and HCC (n=136). HBV was genotyped by multiplex
polymerase chain reaction and subgenotyped by restriction fragment length
polymorphism. HBV mutations were measured by DNA sequencing. HBV genotype C
(68.2%) predominated and genotype B (30.2%) was the second most common. Of
these, C2 (67.5%) was the most prevalent subgenotype, and B2 (30.2%) ranked
second. Thirteen mutations with a frequency >5% were detected. Seven mutation
patterns (C1653T, G1719T, G1730C, T1753C, A1762T, G1764A, and G1799C) were
associated with C2, and four patterns (C1810T, A1846T, G1862T, and G1896A) were
associated with B2. Six patterns (C1653T, G1730C, T1753C, A1762T, G1764A, and
G1799C) were obviously associated with LC, and 10 patterns (C1653T, G1730C,
T1753C, A1762T, G1764A, G1799C, C1810T, A1846T, G1862T, and G1896A) were
significantly associated with HCC compared with CHB. Four patterns (C1810T,
A1846T, G1862T, and G1896A) were significantly associated with HCC compared with
LC. Multivariate regression analyses showed that HBV subgenotype C2 and
C2-associated mutation patterns (C1653T, T1753C, A1762T, and G1764A) were
independent risk factors for LC when CHB was the control, and that B2-associated
mutation patterns (C1810T, A1846T, G1862T, and G1896A) were independent risk
factors for HCC when LC was the control. |
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Keywords: | Hepatitis B virus Genotype Core promoter Precore Mutation Advanced liver disease |
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