不同栽培品种、不同加工方法的温莪术挥发油成分及体外抗肿瘤作用的差异探讨

目的:观察不同栽培品种、不同加工方法的温莪术挥发油的化学成分及其对肿瘤细胞的生长抑制作用的差异。方法:利用MTT法检测同一产地不同栽培品种、不同加工方法的温莪术的挥发油对胃癌肿瘤细胞MGC-803增殖的影响,并用GC-MS检测出挥发油中各成分的相对含量。结果:不同栽培品种、不同加工方法的温莪术挥发油对MGC-803的生长抑制作用存在着明显的差异,当温莪术提取的挥发油为高浓度时,组培品种鲜品对MGC-803的生长抑制最高,为20.2%;当药液浓度为低浓度时,普通品种鲜品对MGC-803的生长抑制最高为18.4%。其所含化学成分也差异明显,如β-水芹烯、双环3.1.1]庚烷,6,6-二甲基-2-亚甲基(1S)是传统鲜品的特征性成分,环己烷,1-乙烯基-1-甲基-2-(1-甲基乙烯基)-4-(1-甲基亚乙基)为普通鲜品的特征性成分;双环3.1.0]六角-2-烯,4-甲基-1-(1-甲基乙基)-,β-蒎烯中,为组培鲜品其特征性成分。结论:不同栽培品种、不同加工方法对其挥发油类成分在含量和组成上的差异较大,对于抑制胃癌肿瘤细胞的增殖作用上也存在明显差异,以组培品种的鲜品与加工品的挥发油对MGC-803的抑制作用最强。

Research on Differences of Composition in Volatile Oil and Anti-Tumor Effect in vitro within Different Cultivated Species and Different Processing Products of Curcuma wenyujin

Objective: To observe the differences of composition in volatile oil and anti-tumor effect in vitro within the different cultivated species and different processing products of Curcuma wenyujin. Method: The volatile oil was extracted by steam distillation, identified by GC-MS, then compared the inhibitive effect on cell line MGC-803 in vitro. Result: Although coming from the same Area, the different cultivated species and different processing products of C. wenyujin have obvious different inhibition on the growth of MGC-803 and have significantly when the volatile oil of C. wenyujin for high concentration, breed of fresh products inhibiting growth of MGC-803 was the highest was 20.2%. When the volatile oil of C. wenyujin for low concentration, common varieties of fresh products inhibiting growth of MGC-803 was the highest was 18.4%. Different chemical compositions in volatile oil. The chemical components are also obvious differences. Such as is beta.-phellandrene bicyclo3.1.1]heptane, 6,6-dimethyl-2-methylene, (1S)-is a characteristic component of traditional fresh product, cyclohexane, 1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene )- is the characteristics of chemical composition of common fresh product, bicyclo3.1.0]hex-2-ene, 4-methyl-1-(1-methylethyl), beta-pinene is the tissue culture of fresh products for characteristic components. Conclusion: Although coming from the same origin, the cultivated species and the processing method of C. wenyujin have great effects on the content and antitumorus activity of the volatile oil. The volatile oil of fresh and processed products breed the strongest inhibitory effect on MGC-803.