γ射线辐照对血小板CD62p表达率的影响

目的探讨γ射线辐照对单采血小板活化及输注效果的影响。方法将30袋单采血小板随机分为对照组和辐照组,分别测定保存前及保存第1、3、5天时血小板活化标志物CD62P的表达率;观察两种血小板输注后出血改善情况及血小板计数的变化情况。结果辐照组与对照组相比,保存前及保存第1、3天时CD62P的表达无显著性差异（P〉0.05）,保存至第5天时,辐照组CD62P的表达较对照组低（P〈0.05）;临床输注后,出血情况均有不同程度改善,两组患者输注血小板后其回收率无明显差异。结论γ射线辐照对单采血小板活化及输注效果均无明显影响,可以达到与输注未经辐照的等量血小板同样的疗效。

Affect of Gamma Irradiation on the expression of CD62P of apheresis platelets

Objective To investigate the affect of gamma irradiation on the activation of apheresis platelets and the clinical effect of the irradiated platelets.Methods Thirty units of apheresis platelets were divided into two groups,one was irradiated with γ-ray at the dose of 30Gy,and the other served as a control.Platelet activation marker CD62P was determined on the day before storage and after 1,3,5 days of storage with Flow Cytometry;the curative effect of γ-ray irradiated apheresis platelet transfusion in patients was observed.Results There were no significant differences between the expressions of CD62P of the irradiated and those of the non-irradiated groups on day0,1,3 of storage（P0.05）.The expression of CD62P of the non-irradiated groups was more than that of the irradiated groups on day5（P0.05）.The PLT counts increased in all patients in 24hrs after transfusion.Bleeding in all patients was improved.There was no significant differences between the two groups.Conclusions Irradiation has no adverse effect on the activation and the treatment efficacy of apheresis platelets.γ-ray irradiated platelet are the similar in increaseing patients＇ peripheral blood PLT counts and preventing bleeding.