Effects of colchicine on the induction of ornithine decarboxylase and its gene expression by the phorbol ester tumour promoter |
| |
| Authors: | T. KONO S. TANIGUCHI N. MIZUNO M. FUKUDA N. MAEKAWA T. HISA M. ISHII S. OTANI T. HAMADA |
| |
| Affiliation: | Department of Dermatology Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka, Japan;Department of Biochemistry, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka, Japan |
| |
| Abstract: | The activity and gene expression of ornithine decarboxylase (ODC, an indicator of tumour promotion) were induced by the phorbol ester tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in mouse skin. In the present study, the effect of colchicine, a microtu-bule-disrupting agent, on ODC activity and its gene expression were investigated. On administration of colchicine (100 μg) intraperitoneally 1·5 h before TPA treatment, ODC activity and ODC mRNA levels stimulated by TPA were suppressed to about 52 and 64%, respectively. These results suggest the involvement of a microtubule or colchicine-sensitive substrate in the signal transduction system for gene expression. |
| |
| Keywords: | |
|
|
