Effects of colchicine on the induction of ornithine decarboxylase and its gene expression by the phorbol ester tumour promoter |
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| Authors: | T KONO S TANIGUCHI N MIZUNO M FUKUDA N MAEKAWA T HISA M ISHII S OTANI T HAMADA |
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| Institution: | Department of Dermatology Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka, Japan;Department of Biochemistry, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka, Japan |
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| Abstract: | The activity and gene expression of ornithine decarboxylase (ODC, an indicator of tumour promotion) were induced by the phorbol ester tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in mouse skin. In the present study, the effect of colchicine, a microtu-bule-disrupting agent, on ODC activity and its gene expression were investigated. On administration of colchicine (100 μg) intraperitoneally 1·5 h before TPA treatment, ODC activity and ODC mRNA levels stimulated by TPA were suppressed to about 52 and 64%, respectively. These results suggest the involvement of a microtubule or colchicine-sensitive substrate in the signal transduction system for gene expression. |
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