Association of Helicobacter pylori infection with Toll‐like receptor‐4 Thr399Ile polymorphism increased the risk of peptic ulcer development in North of Iran |
| |
| Authors: | Mehdi Tourani Maryam Habibzadeh Javad Shokri‐Shirvani Omid Teymournejad Amrollah Mostafazadeh Soraya Khafri Hamid Reza Nouri |
| |
| Institution: | 1. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran;2. Student Research Committee, Babol University of Medical Sciences, Babol, Iran;3. Department of Internal Medicine, Babol University of Medical Sciences, Babol, Iran;4. Faculty of Medical Sciences, Department of Bacteriology, Tarbiat Modares University, Tehran, Iran;5. Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran;6. Biostatistics & Epidemiology Department, Babol University of Medical Sciences, Babol, Iran |
| |
| Abstract: | Toll‐like receptor‐4 (TLR4) polymorphisms may influence host immune response against Helicobacter pylori (H. pylori). This study aimed to investigate whether TLR4 polymorphisms are associated with H. pylori susceptibility and risk of peptic ulcer development or not. The TLR4 + 3725 G/C polymorphism was studied using polymerase chain reaction with confronting two‐pair primers (PCR–CTPP). In addition, TLR4 Asp299Gly and Thr399Ile polymorphisms were evaluated by PCR‐restriction fragment length polymorphism (RFLP). There was no significant difference in TLR4 + 3725 G/C and Asp299Gly genotype frequencies between non‐peptic ulcer (NPUD) and peptic ulcer (PUD) individuals in the context of peptic ulcer development and susceptibility to infection with H. pylori. Nevertheless, a significant association with increased risk for PUD development was observed for polymorphism TLR4 Thr399Ile odds ratio (OR) = 4.2; 95% confidence interval (CI) = 1.35–13.26; p = 0.01]. Correspondingly, TLR4 Thr399Ile polymorphism was associated with H. pylori susceptibility (OR = 0.27; 95% CI = 0.08–0.88; p = 0.04). In addition, TLR4 Thr399Ile polymorphism increased 4.2‐fold, the risk of peptic ulcer development in individuals infected by H. pylori carrying CT + TT genotype. Our results showed that TLR4 Thr399Ile polymorphism along with H. pylori infection may play critical roles in peptic ulcer development in North of Iran. |
| |
| Keywords: |
H pylori
peptic ulcer polymorphism TLR4 |
|
|
