| 子宫内膜癌细胞中RNA干扰Bmi-1基因的实验研究及意义 |
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| 引用本文： | 林小满,谢娜,徐杨. 子宫内膜癌细胞中RNA干扰Bmi-1基因的实验研究及意义[J]. 中国医药科学, 2011, 0(16): 23-24 |
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| 作者姓名： | 林小满 谢娜 徐杨 |
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| 作者单位： | 徐州医学院附属医院妇产科 |
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| 摘 要： | 目的检测子宫内膜癌细胞Ishikawa中RNA干扰Bmi-1基因的实验效果,为进一步研究奠定基础。方法培养子宫内膜癌细胞Ishikawa,采用siRNA干扰技术沉默Ishikawa细胞中Bmi-1基因,采用RT-PCR方法检测干扰后Bmi-1mRNA的表达情况,t检验用于统计学分析。结果 siRNA干扰Bmi-1后,Ishikawa细胞中Bmi-1mRNA的RT-PCR电泳条带灰度比为(0.115±0.011),阴性对照组为(0.287±0.014),两组差异具有统计学意义(P<0.05)。结论 siRNA干扰技术可以降低子宫内膜癌细胞中Bmi-1基因的表达,为进一步研究Bmi-1基因在子宫内膜癌发生发展中的作用奠定基础。
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| 关 键 词： | 子宫内膜癌 Bmi-1 siRNA |
Experimental study and significance of RNA interfering Bmi-1 in endometrial cancer cells |
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| LIN Xiaoman,XIE Na,XU Yang. Experimental study and significance of RNA interfering Bmi-1 in endometrial cancer cells[J]. China Medicine and Pharmacy, 2011, 0(16): 23-24 |
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| Authors: | LIN Xiaoman XIE Na XU Yang |
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| Affiliation: | Department of Gynaecology and Obstetrics,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221000,China |
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| Abstract: | Objective To detect the experimental effect of siRNA interfering Bmi-1 in endometrial cancer cells Ishikawa,the foundation was established for next study. Methods Ishikawa cells were cuLtured,Bmi-1 gene in Ishikawa cells were silenced by siRNA interfering,the expressions of Bmi-1 mRNA was detected by RT-PCR,t test was used to statistically analyze. Results After Bmi-1 in Ishikawa cells were interfered,the gray scale ratio of Bmi-1 mRNA electrophoresis strip in RNAi group was(0.115±0.011) ,negative control group was(0.287±0.014) ,statistically analyzed,the difference was significantly(P < 0.05) . Conclusion The technique of siRNA may decrease the expression of Bmi-1 gene in Ishikawa endometrial cancer cells,the foundation was established for the next study of the effect of Bmi-1 gene in the genesis and development of endometrial cancer. |
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| Keywords: | Endometrial cancer Bmi-1 siRNA |
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