舒林酸抑制胃癌BGC-823细胞生长的实验观察

目的:观察舒林酸对胃癌BGC-823细胞生长的抑制作用,探讨其作用机制.方法:将舒林酸设置不同的作用浓度和作用时间于人胃癌BGC-823细胞共培养.采用MTT比色法检测胃癌细胞抑制率,流式细胞术检测胃癌细胞周期分布,TUNEL法检测细胞凋亡率,免疫组化检测细胞增殖(ki-67)、凋亡抑制基因(survivin)及还氧合酶(COX-2)蛋白的表达.结果:舒林酸使胃癌BGC-823细胞的生长受抑制,出现G0/G1期比例增高,S期比例降低,同时使细胞凋亡率显著上升;而ki-67、survivin及COX-2蛋白表达阳性率显著降低,上述作用均呈时间和剂量依赖性(P＜0.01).结论:舒林酸可抑制胃癌BGC-823细胞生长,其机制涉及影响细胞周期分布、诱导细胞凋亡及抑制COX-2、ki-67及survivin蛋白的表达.

Inhibitory effect of sulindac on the growth of gastric cancer cells in vitro

Objective:To observe the inhibitory effect of sulindac on the growth of gastric cancer cells BGC-823 and its antineoplastic mechanisms.Methods:Human gastric cancer cells BGC-823 were cultured with various concentrations of sulindac for different time.The proliferation of BGC-823 cells was measured by MTT colorimetrie assay;and the cell cycle distribution was examined by flow cytometry.Cell apoptosis was determind by TUNEL.The expressions of ki-67,survivin and COX-2 in cells were detected by immunohistochemical staining.Results:Sulindac inhibited proliferation of BGC-823 cells,increased the proportion of cells in the G_0/G_1 phase,decreased the proportion of cells in the S phase,induced apoptosis of the BGC-823 cell line and decreased the expressions of ki-67,survivin and COX-2 in cells.All the effects were in a time-dependent and dose-dependent manner(P<0.05).Conclusions:Sulindac inhibits the growth of gastric cancer cells BGC-823 and the antitumor mechanism may be related to changing cell cycle distribution,inducing cell apoptosis and inhibiting the expression of COX-2,survivin and ki-67.