Intracellular amino acid concentrations in children with chronic renal insufficiency |
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| Authors: | Joana E. Kist-van Holthe tot Echten Jan G. M. Huijmans Wim C. J. Hop Leo A. H. Monnens Maria C. J. W. de Jong Cornelia M. Noordzij Rembert Slotema Jeroen Nauta Eric D. Wolff |
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| Affiliation: | (1) Department of Pediatrics, Subdivision of Nephrology, Sophia Children's Hospital/University Hospital Rotterdam, Rotterdam, The Netherlands;(2) Department of Pediatrics, Subdivision of Genetic and Inborn Diseases, Sophia Children's Hospital/University Hospital Rotterdam, Rotterdam, The Netherlands;(3) Department of Epidemiology and Biostatistics, Erasmus University Rotterdam, Rotterdam, The Netherlands;(4) Department of Pediatrics, Subdivision of Nephrology, University Hospital Nijmegen, Nijmegen, The Netherlands;(5) Groot Hoefijzerlaan 22, 2244GJ Wassenaar, The Netherlands |
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| Abstract: | We studied amino acid concentrations in granulocytes and plasma of 24 children with chronic renal failure and 15 healthy children. Granulocytes were isolated from 10 ml of blood using a dextran-Ficoll-Hypaque procedure. Intracellular levels of leucine, isoleucine, methionine, phenylalanine, lysine, histidine, tyrosine, and arginine were significantly lower in children with chronic renal failure than healthy children. There were no significant differences in intracellular and plasma amino acid concentrations between children with chronic renal failure on a well-balanced protein-restricted diet and children with chronic renal failure with a normal protein intake. |
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| Keywords: | Chronic renal failure Granulocytes Amino acids Protein restriction |
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