胃癌中MTS1／p16和p53蛋白表达与细胞增殖的关系

应用免疫组织化学S－P法研究50例胃癌组织中p16蛋白和p53的表达情况，并进行增殖细胞核核原（PCNA）检测，计算细胞增殖指数（PI）。结果表明，50例胃镜组织中p16蛋白和p53蛋白阳性率分别为64％和60％。p16蛋白表达：高分化胃癌明显高于低分化胃癌（P＜0.01），淋巴结转移阳性组明显低于阴性组（P＜0.01），PI分组I级明显高于III、IV级 （P＜0.01），存活期＜3年者明显高于阴性组（P＜0.05）。PI分级I级明显低于III、IV级（P＞0.05），存活期＜3年患者明显高于＞3年患者（P＜0.05）。p16低表达与p53过表达之间未见明显相关（P＞0.05）。结果提示p16蛋白低表达和p53蛋白过表达均有促进胃癌细胞增殖作用，且与胃癌细胞分化有关，可作为判断肿瘤生物学行为和患者预后的参考指标之一。

THE RELATION BETWEEN THE EXPRESSION OF MTS1/p16,p53 GENE AND CELL PROLIFERATION OF HUMAN GASTRIC CANCER

Using S P immunohistochemical methods, p16,p53 proteins and PCNA expressions were assessed in 50 specimens of gastric carcinoma. The results showed that the positive expressions for p16 and p53 protein were 64% and 60% respectively. A significant correlation was found between p16 protein low expression, p53 protein overexpression and degree differentiation, lymph node metastasis, and survival rate (P<0.05). But low expression of p16 protein was not correlated with p53 protein overexpression (P>0.05). The results suggested that p16 protein low expression and p53 protein overexpression play an important role in cell proliferation of gastric carcinoma.