| 环氧合酶2抑制剂对肝肿瘤细胞的血管内皮生长因子表达的影响 |
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| 引用本文： | 潘德顺,陈伟强.环氧合酶2抑制剂对肝肿瘤细胞的血管内皮生长因子表达的影响[J].解剖学研究,2009,31(4):256-258. |
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| 作者姓名： | 潘德顺 陈伟强 |
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| 作者单位： | 1. 广东药学院药理教研室,广东,广州,510006
2. 广东药学院病理生理学教研室 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 目的探讨肝肿瘤细胞环氧合酶2(COX-2)和血管内皮生长因子(VEGF)关系。方法实验分为对照组和实验组,培养肝癌细胞株(BEL-7402)作为对照组,培养肝癌细胞株用不同浓度赛来昔布(celeccoxib商品名:西乐葆)处理作为实验组;提取mRNA,用ELISA方法检测细胞培养基中VEGF水平,用RT-PCR分别检测COX-2和VEGF的mRNA。结果正常培养的肝癌细胞能同时检测到COX-2和VEGF的mRNA;COX-2抑制剂赛来昔布能抑制VEGF的表达;COX-2在mRNA水平调控VEGF的表达。结论COX-2在VEGF的分泌与合成中起重要作用,从而影响肿瘤新生血管生成及肿瘤生长。
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| 关 键 词： | 环氧合酶 血管内皮生长因子 血管生成 肝癌 |
Correlation of cyclooxygenase-2 with the expression of vascular endothelial growth factor in hepatocellular carcinoma cell lines |
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| PAN De-shun,CHEN Wei-qiang.Correlation of cyclooxygenase-2 with the expression of vascular endothelial growth factor in hepatocellular carcinoma cell lines[J].Anatomy Research,2009,31(4):256-258. |
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| Authors: | PAN De-shun CHEN Wei-qiang |
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| Institution: | PAN De-shun,CHEN Wei-qiang. Department of Pharmacology,Guangdong College of Pharmacy,Guangzhou 510006 China |
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| Abstract: | Objective To investigate the recationship between cvclooxygenase-2 (COX-2) and expression of vascular endothelial growth factor ( VEGF ) in hepatocellular carcinoma cell line BEL-7402. Methods mRNA was extracted from cultured hepatocellular carcinoma cell lines BEL-7402. The VEGF levels in the culture medium of these cells were measured using ELLSA. The expression of COX-2 and VEGF were identified by RT-PCR. Results The cultured hepatocellular carcinoma cell lines BEL-7402 can express both COX-2 and VEGF at... |
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| Keywords: | Cyclooxygenase-2 Vascular endothelial growth factor Angiogenesis Hepatic cancer |
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