Fusion of mouse-mouse cells to produce hybridomas secreting monoclonal antibody |
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| Authors: | Elizabeth M. Earley Mark C. Osterling |
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| Affiliation: | (1) Virology Division, United States Army Medical Research Institute of Infectious Diseases, Ft. Detrick, 21701 Frederick, Maryland, USA |
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| Abstract: | Summary A procedure is described for fusing and producing large numbers of hybridomas using mouse myeloma cells and immunized mouse spleen cells. The medium used is a modified Dulbeceo's minimum essential medium, supplemented with a low percentage of serum. |
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| Keywords: | cell fusion hybridoma mouse low serum medium |
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