Identical 18S rRNA haplotypes of Hepatozoon canis in dogs and foxes in Brandenburg,Germany |
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| Institution: | 1. Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Berlin, Germany;2. Small Animal Clinic, Freie Universität Berlin, Berlin, Germany;3. LABOKLIN GmbH & Co. KG, Bad Kissingen, Germany;4. Bayer Animal Health GmbH, Monheim, Germany;5. Bayer Vital GmbH, Leverkusen, Germany;6. Landeslabor Berlin-Brandenburg, Frankfurt (Oder), Germany;1. Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, 39660, Republic of Korea;2. College of Veterinary Medicine, Kyungpook National University, Bukgu, Daegu, 41566, Republic of Korea;3. Cardiovascular Research Institute, Kyungpook National University, Junggu, Daegu, 41944, Republic of Korea;1. UCD Veterinary Sciences Centre, Ireland;2. UCD School of Biology and Environmental Science, Ireland;3. UCD School of Agriculture and Food Science and University College Dublin, Ireland;4. MSD, Ireland;5. Moorepark Research Centres, Teagasc, Ireland;6. Athenry Research Centres, Teagasc, Ireland;7. Medical Entomology Group, Emergency Response Department, Public Health England, Salisbury, UK;1. Biology Centre, Institute of Parasitology, Academy of Sciences of the Czech Republic, Brani?ovská 31, 370 05 ?eské Budějovice, Czech Republic;2. Department of Parasitology, Faculty of Science, University of South Bohemia, Brani?ovská 31, 370 05 ?eské Budějovice, Czech Republic;3. Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého t?. 1/3, 612 42 Brno, Czech Republic;4. CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého t?. 1/3, 612 42 Brno, Czech Republic;5. Department of Zoology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina B-1, 842 15 Bratislava, Slovak Republic;6. Department of Ecology and Evolutionary Biology (EBIO), Ramaley N122, Campus Box 334, University of Colorado, Boulder, CO-80309-0334, USA;7. School of Biological Sciences, Flinders University, Adelaide, 5001 South Australia, Australia;8. Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, 5000 South Australia, Australia;9. Department of Biology, Faculty of Science, Razi University, Baghabrisham 67149, Kermanshah, Iran;10. Department of Biology, University of Badji Mokhtar, BP 12, El Hadjar, 23000 Annaba, Algeria;1. Department of Veterinary Medicine, University of Bari, Bari, Italy;2. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy;3. Osservatorio Faunistico Venatorio, Campania Region, Naples, Italy;4. CEITEC - Central European Institute of Technology, University of Veterinary and Pharmaceutical Sciences, Brno, Brno, Czech Republic;5. Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic;6. Faculty of Medicine in Pilsen, Biomedical Center, Charles University, alej Svobody 1655/76, 32300, Plzeň, Czech Republic;7. Istituto Zooprofilattico Sperimentale del Mezzogiorno, 80055, Portici, Italy;8. Biology Centre Czech Academy of Sciences, Institute of Parasitology, Branisovska 31, 37005, Ceske Budejovice, Czech Republic;9. Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic;10. Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan, Iran |
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| Abstract: | Hepatozoon canis is a blood parasite of the suborder Adeleorina infecting wild and domestic canids. Transmission occurs by oral uptake of Rhipicephalus sanguineus sensu lato vector ticks infected with H. canis, but vertical transmission is also assumed to be possible. In German foxes, a high prevalence of H. canis has previously been reported despite the fact that R. sanguineus s.l. is not endemic. In the absence of knowledge about local transmission pathways, foxes should be considered to be possible reservoirs of H. canis and contribute to infection of domestic dogs. The present study aimed to determine how often foxes and dogs are infected in Brandenburg (Germany) and if identical or different H. canis 18S rRNA haplotypes are found in these host species. Hepatozoon spp. were detected by PCR in 46/1050 (4.4 %) of dog blood and 176/201 (77.6 %) of fox spleen samples from Brandenburg. Sequencing of 19 dog and 56 fox samples identified all as H. canis. For nine positive dogs, owners stated that they had never left Germany suggesting that autochthonous transmission occurs not only in foxes but also in dogs. Sequences for seven of these possible autochthonous cases were obtained and six were identical to the predominant haplotype found in the foxes. Haplotype network analysis confirmed that many dogs, including some without travel history, carried the same or very similar 18S rRNA haplotypes as the foxes suggesting that both hosts participate in the same epidemiological cycle. |
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| Keywords: | PCR Dog Fox Germany Tick-borne pathogen |
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