Molecular epidemiology of Rickettsia sp. and Coxiella burnetii collected from Hyalomma asiaticum in Bactrian camels (Camelus bactrianus) in inner Mongolia of China |
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| Institution: | 1. College of Animal Science, Inner Mongolia Agricultural University, No. 306 Zhaowuda Road, Hohhot City, Inner Mongolia 010018, China;2. College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306 Zhaowuda Road, Hohhot City, Inner Mongolia 010018, China;1. Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan;2. Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan;1. IHU-Méditerranée Infection, Marseille, France;2. Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France;3. Department of Biology, University Yahya Fares of Medea, Algeria;4. National Veterinary High School, Algiers, Algeria;5. Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France;6. PADESCA Laboratory, Veterinary Science Institute, University Constantine 1, 25100, El Khroub, Algeria;7. Laboratoire de Biodiversité et Environnement, Faculté des Sciences Biologique, Université des Sciences et de la Technologie Houari Boumediene, Alger, Algeria;1. Parasitology Division, National Veterinary Research Institute (NVRI), PMB 01 Vom, Plateau State, Nigeria;2. Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel;3. Parasitology Unit, Department of Microbiology and Molecular Genetics, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem, Israel;1. Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech Republic;2. National University of Mongolia, Ulaanbaatar, Mongolia;3. Institute of General and Experimental Biology of Mongolian Academy of Sciences, Ulaanbaatar, Mongolia;1. Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, 54600, Lahore, Pakistan;2. District Diagnostic Laboratory, Livestock & Dairy Development Department, 42200, Mianwali, Pakistan;3. Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, 54600, Lahore, Pakistan;4. Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, 54600, Lahore, Pakistan;5. University College of Veterinary and Animal Sciences, Islamia University of Bahawalpur-Pakistan, Pakistan;6. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, PR China;1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080-8555, Hokkaido, Japan;2. Parasitology Laboratory, Veterinary College, Xinjiang Agricultural University, Urumqi, 830011, Xinjiang, China;3. Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt |
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| Abstract: | The purpose of the present study was to determine the presence of Rickettsia sp. and Coxiella burnetii in Hyalomma asiaticum collected from Bactrian camels (Camelus bactrianus) in Inner Mongolia of China. A total of 385 H. asiaticum adults collected from Bactrian camels from Alxa in Inner Mongolia from 2017 to 2018 were examined using PCR combined with sequencing. The results indicated that 18 (4.7 %) and 5 (1.3 %) samples tested positive for Rickettsia and C. burnetii, respectively. The corresponding nucleotide identities among detected genes ranged between 99.7 % and 100 % for the sequences of 17 kD and ompB from Rickettsia and 16S rRNA and FtsX from C. burnetii. Two representative strains—Rickettsia IMA-1 strain and C. burnetii IMA-1 strain—were used for subsequent analysis. NCBI BLAST and phylogenetic analyses demonstrated that the detected strain Rickettsia IMA-1 may represent a novel species of spotted fever group (SFG) rickettsiae. The C. burnetii IMA-1 strain was grouped with Coxiella burnetii str. RSA439 (GenBank: CP040059.1). In addition, the Rickettsia sp. was successfully isolated from the ticks in Vero cells incubated at 28 °C. These findings indicate that the H. asiaticum collected off Bactrian camels in Inner Mongolia carried SFG Rickettsia species and C. burnetii and could contribute to the etiology of febrile illness in animals and humans. |
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| Keywords: | Molecular epidemiology Bactrian camel |
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