Reactivity of a monoclonal antibody recognicing an estrogen receptor regulated glycoprotein in relation to lectin histochemistry in breast cancer |
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Authors: | Markku Helle Kai Krohn |
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Institution: | (1) Department of Pathology, Mikkeli Central Hospital, SF-50100 Mikkeli, Finland;(2) Institute of Biomedical Sciences, University of Tampere, SF-Tampere, Finland |
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Abstract: | Summary We have raised monoclonal antibodies against human milk fat globule membrane antigens and previously shown that one of them, called III D 5, recognises a glycoprotein associated with estrogen receptor activity of breast cancer. In immunoblotting it was shown that the molecule in human milk exclusively stained with III D 5 also binds peanut agglutinin (PNA) and Ricinus communis. In this study we correlate the staining of III D 5 and binding of lectins to tissue sections fixed in formalin and embedded in paraffin. Similar rections were seen only with III D 5 and PNA. Our results suggest that III D 5 and PNA detect overlapping antigenic epitopes in mammary carcinoma. This is in keeping with previous results that PNA or III D 5 reactivity is correlated with estrogen receptor status of breast cancer. |
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Keywords: | Monoclonal antibody Estrogen receptor Lectins Breast cancer |
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