实验性精索静脉曲张睾丸生精功能障碍的研究

为探讨精索静脉曲张睾丸生精功能暨不育症的发生机制,采用家兔复制精索静脉曲张动物模型,观察睾丸组织LPO、cAMP的质量摩尔浓度、精液量和精子密度的变化及其相互关系。发现精索静脉曲张组(VCG)左右睾丸组织LPO质量摩尔浓度均显著高于模拟手术对照组(SOG);VCG双侧cAMP质量摩尔浓度均显著低于SOG;VCG精子密度明显低于SOG(P<0.01)。而且睾丸组织LPO与cAMP呈明显负相关,cAMP与精子密度呈正相关。结果提示:精索静脉曲张时睾丸组织的脂质过氧化损伤导致细胞内cAMP低下,影响正常代谢,以及高水平LPO直接损伤睾丸生殖细胞致使睾丸生精功能减弱。

Study of Dysfunction of Testis Spermatogenesis in Rabbits with Experimental Varicocele

The objective was to explore the mechanism of the testicle dysfunction-infertility derived from varicocele. Varicocele models in rabbits were used to observe the changes of LPO contents and cAMP levels in testis tissue, sperm volumes, sperm density and its relationships. Testis LPO contents of left and right sides in VCG were significantly higher than that in SOG; cAMP levels of bilateral sides in VCG were much lower than that in SOG; the sperm density of VCG was much lower than that of SOG; but there was no difference in sperm volumes between VCG and SOG; there was a negative correlation between LPO contents and cAMP levels, and a notable positive correlation between cAMP levels and sperm density. The higher level of lipid peroxidation in the testicle tissue of varicocele caused the decrease of cAMP levels in the cells, which couldn' t carry on efficiently the normal metabolism of function, especially the spermatogenesis, and did direct damage to the germ cells of the testes, resulting in the sper-matogenous disorder of the testes.