| 用光镜直接从培养细胞中检测细胞凋亡的研究 |
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| 引用本文： | 熊琛 郭怀兰 等. 用光镜直接从培养细胞中检测细胞凋亡的研究[J]. 郧阳医学院学报, 1997, 16(2): 66-67 |
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| 作者姓名： | 熊琛 郭怀兰 等 |
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| 作者单位： | 郧阳医学院微生物学教研室 |
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| 摘 要： | 目的：观察细胞培养中凋亡细胞的形态。方法：取４℃环境下不同时间培养的小鼠胸腺细胞，与台盼蓝混合制成悬液片，用光镜观察。结果：可见凋亡细胞核形态改变及凋亡小体。结论：光镜下依据典型凋亡细胞形态变化可确认凋亡。
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| 关 键 词： | 凋亡 光镜 |
Detection of the Apoptosis among of Cells in Cultivation under an Optical Microscope |
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| Abstract: | Objective:To observe the morphologic change of apoptosis in cell cultivation.Methods:Suspension slides of murine thymocytes cultured at temperature of 4 ℃ and trypan blue and then observed under a light microscope at different time.Results:Morphologic changes of apoptosis nuclei,especially the apoptosis bodies,could be detected under a light microscope.Conclusions:Apoptosis can be indentified depending on the typical changes in nuclear forms with an optical microscope. |
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| Keywords: | apoptosis light microscope |
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