缺铁性贫血患儿红细胞密度及红细胞超氧化物歧化酶的相应分析

采用密度梯度离心法将12例缺铁性贫血(IDA)患儿和15例正常儿童的红细胞离心分离为各1/3上、中、下三层,并采用煌焦油蓝生理盐水法。邻苯三酚自氧化抑制法分别测定其红细胞的Ret分数和E-SOD的活力,对照组和病例组的Ret上中下三层分别为0.020,0.009,0.002和0.035,0.013,0.002(P<0.01,P<0.05和P>0.05);两组的E-SOD上中下三层分别为6349,5721,5301和6850,6165,5024(P<0.05,P<0.05和P>0.05)。结果提示,上层为年轻红细胞,下层细胞逐渐衰老,IDA上层E-SOD受氧自由基影响,代偿性活力增高,IDA红细胞包含了膜LPO增加和E-SOD代偿消耗的综合病理过程。

Corresponding Analysis of Erythrocyte Density and Erythrocyte Superoxide Dismutase in Children with Iron Deficiency Anemia

In this study, red cells of 15 normal children and 12 IDA children were stratified into 3 layers respectively using density gradient centrifugation. Each of the layers consisted of 1/3 part of the sample. Ret fractions and E-SOD validity were measured by using normal saline brilliant cresyl blue method and O-trihydroxy benzene inhibition method respectively The top,middle and bottom layer Ret fractions of control group and IDA group are 0.020, 0.009, 0.002 and 0.035, 0.013, 0.002 respctively (P<0.01,P<0.05, P>0.05);E-SOD of the three layers from top to bottom of the control group and IDA group are 6349, 5721,5301 and 6850,6165,5024 respectively (P<0.05, P<0.05 and P>0.05). The results suggest that the top layers are young cells,and the bottom layers are aging cells.Affected by oxygen free radicals, the E-SOD validity of IDA top layer is compen-satively high.IDA red cells experienced the pathological process of membrane LPO increasing and E-SOD consumption.