| 猪苓子实体培育猪苓菌丝的环境因素研究 |
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| 引用本文： | 周莉英,王西芳,赵萌,杨强. 猪苓子实体培育猪苓菌丝的环境因素研究[J]. 辽宁中医杂志, 2010, 0(9): 1816-1818 |
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| 作者姓名： | 周莉英 王西芳 赵萌 杨强 |
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| 作者单位： | [1]陕西中医学院药学院,陕西咸阳712046 [2]西安市中心医院,陕西西安710000 [3]勉县本草研究所,陕西勉县724200 |
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| 基金项目： | 陕西省教育厅科研基金项目（06JK317） |
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| 摘 要： | 目的:观察人工诱导猪苓子实体培育猪苓菌丝的最佳环境因素,为筛选优良菌种提供依据。方法:用人工诱导的猪苓子实体,采取菌丝分离法接种在适宜的培养基上,观察温度、pH值,光照、培养时间对菌丝形成的影响。结果:沙氏琼脂培养基,温度22℃,pH值5.8,黑暗培养,培养25天时菌丝生长最好。结论:人工诱导的猪苓子实体可以培育优良的猪苓菌种。
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| 关 键 词： | 猪苓子实体 菌丝 环境因素 |
Studies on Environmental Factors to Breed Hyphae from Sporophore of Grifola Umbellate |
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| ZHOU Li-ying,WANG Xi-fang,ZHAO Meng,YANG Qiang. Studies on Environmental Factors to Breed Hyphae from Sporophore of Grifola Umbellate[J]. Liaoning Journal of Traditional Chinese Medicine, 2010, 0(9): 1816-1818 |
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| Authors: | ZHOU Li-ying WANG Xi-fang ZHAO Meng YANG Qiang |
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| Affiliation: | 1. Pharmaceutical Department of Shaanxi College of traditional Chinese Medicine,Xianyang 712046,Shaanxi,China; 2. Central Hospital of Xi＇an City Xi＇an 710000,Shaanxi,China; 3. Mianxian Herbal Medicine Institute,Mianxian 724200,Shaanxi,China) |
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| Abstract: | Objective：To observe the best environmental factors breeding hypha from the Artificially induced sporophore of Grifola umbellate,To provide excellent screening strains. Methods：Using artificially induced sporophore,hypha isolation method ,ob-serve the temperature、pH value、sunlight、incubation time influence on forming hypha. Results：Hypha growth is best on Sabourauds Agar,in the environment of temperature 22℃,pH VALUE 5. 8,no sunlight,25d training. Conclusion：Artificially induced sporophore can cultivate good strain of Grifola umbellate. |
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| Keywords: | sporophore of Grifola umbellate hypha environmental factors |
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