熄风定颤汤对PD模型大鼠单氨类神经递质的影响

目的:观察熄风定颤汤(以下简称中药)对PD模型大鼠旋转行为和纹状体单氨类神经递质的影响,探讨其可能作用机制。方法:采用6-OHDA偏侧纹状体两点注入法制备PD模型大鼠,随机分为模型组、美多巴组、中药高、中、低剂量组,并设假手术组为对照组,灌胃治疗4周。观察各组大鼠治疗前后APO诱导的行为学变化及对纹状体单胺类神经递质的影响。结果:美多巴组灌胃后2周旋转行为有所下降(P<0.01),但4周后又有所增加(P>0.05);中药高、低剂量组灌胃后4周后旋转行为有明显改善(P<0.05,P<0.05);中药中剂量组灌胃后2周、4周后旋转行为均有明显改善(P<0.05,P<0.01)。美多巴组、中药高、中、低剂量组大鼠损毁侧纹状体DA含量、HVA、DOPAC含量均明显增加,其中中剂量组增加最明显,分别比损毁对侧下降了21.78%、23.53%、3.52%,与损毁对侧、假手术组损毁侧差异不显著(P均>0.05),与模型组大鼠DA和DOPAC相比差异显著(P<0.01,P<0.05)。结论:熄风定颤汤可明显改善PD模型大鼠的旋转行为、增加纹状体内DA及其代谢产物的含量,以中剂量组效果最明显。

Research on the Effects of Xifengdingchan Tang on Monoaminergic Neurotransmitter in PD Model Rats

Objective：To observe the effect of Xifengdingchan tang on rotational behavior,striatal monoaminergic neurotransmitter of PD rats and analyze its effective mechanism. Methods ：PD model rats were made by injecting 6 -OHDA twopoint into unilateral striatum, randomly divided into model group, Madopar group, herbal medicine high, medium and low dose group, sham - operated group established for the control group ,treatment lasted for 4 weeks. The ethological change of PD model rats induced by apomorphine （APO） and stfiatal monoaminergic neurotransmitter were observed before treatment and 4 weeks＇ treatment. Results： The rotational behavior of PD in Madopar group decreased after 2 weeks＇ treatment （ P 〈 0.01 ） , but increased after 4 weeks＇ treatment （ P 〉 0.05 ） ; in high and low dose group improved obviously after 4 weeks＇ treatment （ P 〈 0. 05, P 〈 0. 05 ） ; in medium dose group improved obviously both after 2 and 4 weeks＇ treatment （ P 〈 0. 05, P 〈 0. 01 ）. The content of dopamine （DA） , homovanillic acid（HVA） ,dihydroxyphenylacetie acid（DOPAC） in damaged lateral were all markedly increased in Madopar group, high,medium and low dose group;the medium dose group increased the most obviously, respectively decreased 21.78% ,23. 53% ,3.52% compared with the contralateral;there was no significant difference compared with the eontralateral and sham group （ P 〉 0.05 ） ; difference of DA and DOPAC were significant compared with model group, （ P 〈 0. 01, P 〈 0. 05 ）. Conclusion ： Xifengdingchan tang could significantly improve the rotational behavior in PD rats and increase the content of striatal DA and its metabolites,medium dose group was most obviously.