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早孕绒毛Y基因的快速诊断
引用本文:钟南,郭申,刘希贤.早孕绒毛Y基因的快速诊断[J].华中科技大学学报(医学版),1993(2).
作者姓名:钟南  郭申  刘希贤
作者单位:同济医科大学基础医学研究所医学遗传研究室 (钟南,郭申),同济医科大学基础医学研究所医学遗传研究室(刘希贤)
摘    要:许多伴性遗传疾病需在孕早期进行胎儿性别鉴定,报告一种不经Southern转移的分子杂交方法,即利用凝胶原位杂交法对早孕绒毛Y基因进行快速诊断。该方法是在提取孕8周绒毛DNA,经微型琼脂糖凝胶电泳后,直接将凝胶抽干成凝胶膜,尔后以Y染色体3.4 kb DNA片段为特异性探针,直接在凝胶膜上进行分子杂交。本文成功地在48 h内对 1例DMD携带者早孕绒毛的胎儿性别进行了基因诊断。

关 键 词:产前诊断  基因探测  绒毛  性别  分子杂交

Fast Diagnosis of Y-gene from Early Gestation Villi
Zhong Nan,Guo Shen,Liu Xixian.Fast Diagnosis of Y-gene from Early Gestation Villi[J].Journal of Huazhong University of Science and Technology(Health Sciences),1993(2).
Authors:Zhong Nan  Guo Shen  Liu Xixian
Institution:Zhong Nan,Guo Shen,Liu Xixian Department of Medical Genetics,Faculty of Basic Medical Sciences
Abstract:The fetal sex should be detected in the early gestation for many inherited sex-linked diseases. One method of molecular hybridization, hybridized directly on agarose minigel without Southern transfer, was reported here to diagnose Y-gene from early gestational villi. By use of this method, DNA was extracted from early gestational villi (8 w), the agarose gel was pumped into a gel-membrane after electrophoresis, and then the sample DNA on gel membrane was hybridized direclty with specific PY3.4 probe. A fetal sex was successfully diagnosed by detecting DNA of early chorionic villi from the DMD carrier in 48 h.
Keywords:prenatal diagnosis  gene detection  chorionic villi  sex  molecular hybridization
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